Human nicotinamide N-methyltransferase gene: Molecular cloning, structural characterization and chromosomal localization

Saime Aksoy, Brigitte F. Brandriff, Aviva Ward, Peter F.R. Little, Richard M. Weinshilboum

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Genomic DNA clones for nicotinamideN-methyltransferase (NNMT), an enzyme that catalyzes drug and xenobiotic metabolism, were isolated from a human chromosome 11-specific DNA library. Study of one of those clones, when combined with PCR-based experiments performed with human genomic DNA, made it possible to determine the structure of the human NNMT gene. The gene was approximately 16.5 kb in length and consisted of 3 exons and 2 introns. Transcription initiation for the NNMT gene occurred 105-109 nucleotides 5′-upstream from the cDNA translation initiation codon on the basis of the results of both primer extension and 5′-rapid amplification of cDNA ends.NNMTmapped to chromosome band 11q23.1 by fluorescencein situhybridization.

Original languageEnglish (US)
Pages (from-to)555-561
Number of pages7
JournalGenomics
Volume29
Issue number3
DOIs
StatePublished - Oct 1995

ASJC Scopus subject areas

  • Genetics

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