Human nicotinamide N-methyltransferase gene: Molecular cloning, structural characterization and chromosomal localization

S. Aksoy, B. F. Brandriff, A. Ward, P. F R Little, Richard M Weinshilboum

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Genomic DNA clones for nicotinamide N-methyltransferase (NNMT), an enzyme that catalyzes drug and xenobiotic metabolism, were isolated from a human chromosome 11-specific DNA library. Study of one of those clones, when combined with PCR-based experiments performed with human genomic DNA, made it possible to determine the structure of the human NNMT gene. The gene was approximately 16.5 kb in length and consisted of 3 exons and 2 introns. Transcription initiation for the NNMT gene occurred 105109 nucleotides 5'- upstream from the cDNA translation initiation codon on the basis of the results of both primer extension and 5'-rapid amplification of cDNA ends. NNMT mapped to chromosome band 11q23.1 by fluorescence in situ hybridization.

Original languageEnglish (US)
Pages (from-to)555-561
Number of pages7
JournalGenomics
Volume29
Issue number3
DOIs
StatePublished - 1995

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Nicotinamide N-Methyltransferase
Molecular Cloning
Complementary DNA
Clone Cells
Genes
Chromosomes, Human, Pair 11
Initiator Codon
DNA
Human Chromosomes
Xenobiotics
Fluorescence In Situ Hybridization
Gene Library
Introns
Exons
Nucleotides
Chromosomes
Polymerase Chain Reaction
Enzymes
Pharmaceutical Preparations
human NNMT protein

ASJC Scopus subject areas

  • Genetics

Cite this

Human nicotinamide N-methyltransferase gene : Molecular cloning, structural characterization and chromosomal localization. / Aksoy, S.; Brandriff, B. F.; Ward, A.; Little, P. F R; Weinshilboum, Richard M.

In: Genomics, Vol. 29, No. 3, 1995, p. 555-561.

Research output: Contribution to journalArticle

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