TY - JOUR
T1 - Human nicotinamide N-methyltransferase gene
T2 - Molecular cloning, structural characterization and chromosomal localization
AU - Aksoy, Saime
AU - Brandriff, Brigitte F.
AU - Ward, Aviva
AU - Little, Peter F.R.
AU - Weinshilboum, Richard M.
N1 - Funding Information:
We thank Luanne Wussow for her assistance with the preparation of the manuscript, Dr. Eric Wieben and Chengtao Her for their advice, and Denise Lee for performing the FISH analysis. This work was supported in part by NIH Grants RO1 GM28157 and RO1 GM35720 (R.M.W.). The work performed by the Lawrence Livermore National Laboratory was under the auspices of U.S. Department of Energy Contract W-7405-Eng-48. The work performed in PFRL's laboratory was supported by grants from the Human Genome Mapping Directed Program of the UK Medical Research Council and by Grant GENE-CT930057 from the European Commission.
PY - 1995/10
Y1 - 1995/10
N2 - Genomic DNA clones for nicotinamideN-methyltransferase (NNMT), an enzyme that catalyzes drug and xenobiotic metabolism, were isolated from a human chromosome 11-specific DNA library. Study of one of those clones, when combined with PCR-based experiments performed with human genomic DNA, made it possible to determine the structure of the human NNMT gene. The gene was approximately 16.5 kb in length and consisted of 3 exons and 2 introns. Transcription initiation for the NNMT gene occurred 105-109 nucleotides 5′-upstream from the cDNA translation initiation codon on the basis of the results of both primer extension and 5′-rapid amplification of cDNA ends.NNMTmapped to chromosome band 11q23.1 by fluorescencein situhybridization.
AB - Genomic DNA clones for nicotinamideN-methyltransferase (NNMT), an enzyme that catalyzes drug and xenobiotic metabolism, were isolated from a human chromosome 11-specific DNA library. Study of one of those clones, when combined with PCR-based experiments performed with human genomic DNA, made it possible to determine the structure of the human NNMT gene. The gene was approximately 16.5 kb in length and consisted of 3 exons and 2 introns. Transcription initiation for the NNMT gene occurred 105-109 nucleotides 5′-upstream from the cDNA translation initiation codon on the basis of the results of both primer extension and 5′-rapid amplification of cDNA ends.NNMTmapped to chromosome band 11q23.1 by fluorescencein situhybridization.
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U2 - 10.1006/geno.1995.9966
DO - 10.1006/geno.1995.9966
M3 - Article
C2 - 8575745
AN - SCOPUS:0028845483
SN - 0888-7543
VL - 29
SP - 555
EP - 561
JO - Genomics
JF - Genomics
IS - 3
ER -