Human indolethylamine N-methyltransferase: cDNA cloning and expression, gene cloning, and chromosomal localization

Michael A. Thompson, Eunpyo Moon, Ung Jin Kim, Jingping Xu, Michael J. Siciliano, Richard M Weinshilboum

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Abstract

Indolethylamine N-methyltransferase (INMT) catalyzes the N-methylation of tryptamine and structurally related compounds. We recently cloned and characterized the rabbit INMT cDNA and gene as a step toward cloning the cDNA and gene for this enzyme in humans. We have now used a PCR-based approach to clone a human INMT cDNA that had a 792-bp open reading frame that encoded a 263-amino-acid protein 88% identical in sequence to rabbit INMT. Northern blot analysis of 35 tissues showed that a 2.7-kb INMT mRNA species was expressed in most tissues. When the cDNA was expressed in COS-1 cells, the recombinant enzyme catalyzed the methylation of tryptamine with an apparent K(m) value of 2.9 mM. The human cDNA was then used to clone the human INMT gene from a human genomic BAC library. The gene was 5471 bp in length, consisted of three exons, and was structurally similar to the rabbit INMT gene as well as genes for nicotinamide N-methyltransferase and phenylethanolamine N-methyltransferase in several species. All INMT exon- intron splice junctions conformed to the 'GT-AG' rule, and no canonical TATA or CAAT sequences were present within the 5'-flanking region of the gene. Human INMT mapped to chromosome 7p15.2-p15.3 on the basis of both PCR analysis and fluorescence in situ hybridization. Finally, two possible single nucleotide polymorphisms were identified within exon 3, both of which altered the encoded amino acid. The cloning and expression of a human INMT cDNA, as well as the cloning, structural characterization, and mapping of its gene represent steps toward future studies of the function and regulation of this methyltransferase enzyme in humans.

Original languageEnglish (US)
Pages (from-to)285-297
Number of pages13
JournalGenomics
Volume61
Issue number3
DOIs
StatePublished - Nov 1 1999

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tryptamine N-methyltransferase
Organism Cloning
Complementary DNA
Gene Expression
Genes
Exons
Rabbits
Methylation
Nicotinamide N-Methyltransferase
Enzymes
Clone Cells
Phenylethanolamine N-Methyltransferase
Amino Acids
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Genetics

Cite this

Human indolethylamine N-methyltransferase : cDNA cloning and expression, gene cloning, and chromosomal localization. / Thompson, Michael A.; Moon, Eunpyo; Kim, Ung Jin; Xu, Jingping; Siciliano, Michael J.; Weinshilboum, Richard M.

In: Genomics, Vol. 61, No. 3, 01.11.1999, p. 285-297.

Research output: Contribution to journalArticle

Thompson, Michael A. ; Moon, Eunpyo ; Kim, Ung Jin ; Xu, Jingping ; Siciliano, Michael J. ; Weinshilboum, Richard M. / Human indolethylamine N-methyltransferase : cDNA cloning and expression, gene cloning, and chromosomal localization. In: Genomics. 1999 ; Vol. 61, No. 3. pp. 285-297.
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abstract = "Indolethylamine N-methyltransferase (INMT) catalyzes the N-methylation of tryptamine and structurally related compounds. We recently cloned and characterized the rabbit INMT cDNA and gene as a step toward cloning the cDNA and gene for this enzyme in humans. We have now used a PCR-based approach to clone a human INMT cDNA that had a 792-bp open reading frame that encoded a 263-amino-acid protein 88{\%} identical in sequence to rabbit INMT. Northern blot analysis of 35 tissues showed that a 2.7-kb INMT mRNA species was expressed in most tissues. When the cDNA was expressed in COS-1 cells, the recombinant enzyme catalyzed the methylation of tryptamine with an apparent K(m) value of 2.9 mM. The human cDNA was then used to clone the human INMT gene from a human genomic BAC library. The gene was 5471 bp in length, consisted of three exons, and was structurally similar to the rabbit INMT gene as well as genes for nicotinamide N-methyltransferase and phenylethanolamine N-methyltransferase in several species. All INMT exon- intron splice junctions conformed to the 'GT-AG' rule, and no canonical TATA or CAAT sequences were present within the 5'-flanking region of the gene. Human INMT mapped to chromosome 7p15.2-p15.3 on the basis of both PCR analysis and fluorescence in situ hybridization. Finally, two possible single nucleotide polymorphisms were identified within exon 3, both of which altered the encoded amino acid. The cloning and expression of a human INMT cDNA, as well as the cloning, structural characterization, and mapping of its gene represent steps toward future studies of the function and regulation of this methyltransferase enzyme in humans.",
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