Human HLA-DQβ chain presents minor lymphocyte stimulating locus gene products and clonally deletes TCR Vβ6+, Vβ8.1+ T cells in single transgenic mice

Paul Zhou, Gary D. Anderson, Suresh Savarirayan, Hidetoshi Inoko, Chella S. David

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Minor lymphocyte stimulating locus (Mls) gene products in association with mouse major histocompatibility complex (MHC) class II molecules are known to determine the repertoire of T-cell receptor (TCR) in mature T cells. In order to test whether human class II molecules can present mouse Mls, HLA-DQβ transgenic mice were generated. The expression and function of the DQβ transgene were studied in the progeny of one selected founder which was H-2f and H-2E negative. In these mice, DQβ molecules pairing with mouse Aα chain and invariant chain are expressed on the cell surface in a tissue-specific manner. When the DQβ gene was bred into the Mls-1a strain DBA/1 (H-2q), T cells bearing Vβ6 and Vβ8.1 TCR were clonally deleted in the thymus of DQβ + transgenics but not in DQβ-negative full sibs. Thus, the data presented here clearly demonstrate that the human MHC DQβ chain can present Mls in the clonal deletion of T cells. Our results also suggest the requirement for an interaction between CD4 and class II molecules (α chain) for clonal deletion of T cells to occur.

Original languageEnglish (US)
Pages (from-to)47-56
Number of pages10
JournalHuman Immunology
Volume31
Issue number1
DOIs
StatePublished - 1991

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HLA-DQ Antigens
T-Cell Antigen Receptor
Transgenic Mice
Clonal Deletion
Lymphocytes
T-Lymphocytes
Major Histocompatibility Complex
Genes
Transgenes
Thymus Gland

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Human HLA-DQβ chain presents minor lymphocyte stimulating locus gene products and clonally deletes TCR Vβ6+, Vβ8.1+ T cells in single transgenic mice. / Zhou, Paul; Anderson, Gary D.; Savarirayan, Suresh; Inoko, Hidetoshi; David, Chella S.

In: Human Immunology, Vol. 31, No. 1, 1991, p. 47-56.

Research output: Contribution to journalArticle

Zhou, Paul ; Anderson, Gary D. ; Savarirayan, Suresh ; Inoko, Hidetoshi ; David, Chella S. / Human HLA-DQβ chain presents minor lymphocyte stimulating locus gene products and clonally deletes TCR Vβ6+, Vβ8.1+ T cells in single transgenic mice. In: Human Immunology. 1991 ; Vol. 31, No. 1. pp. 47-56.
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