Minor lymphocyte stimulating locus (Mls) gene products in association with mouse major histocompatibility complex (MHC) class II molecules are known to determine the repertoire of T-cell receptor (TCR) in mature T cells. In order to test whether human class II molecules can present mouse Mls, HLA-DQβ transgenic mice were generated. The expression and function of the DQβ transgene were studied in the progeny of one selected founder which was H-2f and H-2E negative. In these mice, DQβ molecules pairing with mouse Aα chain and invariant chain are expressed on the cell surface in a tissue-specific manner. When the DQβ gene was bred into the Mls-1a strain DBA/1 (H-2q), T cells bearing Vβ6 and Vβ8.1 TCR were clonally deleted in the thymus of DQβ+ transgenics but not in DQβ-negative full sibs. Thus, the data presented here clearly demonstrate that the human MHC DQβ chain can present Mls in the clonal deletion of T cells. Our results also suggest the requirement for an interaction between CD4 and class II molecules (α chain) for clonal deletion of T cells to occur.
ASJC Scopus subject areas
- Immunology and Allergy