Human histone acetyltransferase 1 protein preferentially acetylates H4 histone molecules in H3.1-H4 over H3.3-H4

Hui Zhang, Junhong Han, Bin Kang, Rebecca Burgess, Zhiguo Zhang

Research output: Contribution to journalArticle

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Abstract

In mammalian cells, canonical histone H3 (H3.1) and H3 variant (H3.3) differ by five amino acids and are assembled, along with histone H4, into nucleosomes via distinct nucleosome assembly pathways. H3.1-H4 molecules are assembled by histone chaperone CAF-1 in a replication-coupled process, whereas H3.3-H4 are assembled via HIRA in a replication-independent pathway. Newly synthesized histone H4 is acetylated at lysine 5 and 12 (H4K5,12) by histone acetyltransferase 1 (HAT1). However, it remains unclear whether HAT1 and H4K5,12ac differentially regulate these two nucleosome assembly processes. Here, we show that HAT1 binds and acetylates H4 in H3.1-H4 molecules preferentially over H4 in H3.3-H4. Depletion of Hat1, the catalytic subunit of HAT1 complex, results in reduced H3.1 occupancy at H3.1-enriched genes and reduced association of Importin 4 with H3.1, but not H3.3. Finally, depletion of Hat1 or CAF-1p150 leads to changes in expression of a H3.1-enriched gene. These results indicate that HAT1 differentially impacts nucleosome assembly of H3.1-H4 and H3.3-H4.

Original languageEnglish (US)
Pages (from-to)6573-6581
Number of pages9
JournalJournal of Biological Chemistry
Volume287
Issue number9
DOIs
StatePublished - Feb 24 2012

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Histones
Nucleosomes
Molecules
Proteins
Histone Chaperones
Genes
Karyopherins
Lysine
Catalytic Domain
Cells
histone acetyltransferase type B complex
Amino Acids

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Human histone acetyltransferase 1 protein preferentially acetylates H4 histone molecules in H3.1-H4 over H3.3-H4. / Zhang, Hui; Han, Junhong; Kang, Bin; Burgess, Rebecca; Zhang, Zhiguo.

In: Journal of Biological Chemistry, Vol. 287, No. 9, 24.02.2012, p. 6573-6581.

Research output: Contribution to journalArticle

Zhang, Hui ; Han, Junhong ; Kang, Bin ; Burgess, Rebecca ; Zhang, Zhiguo. / Human histone acetyltransferase 1 protein preferentially acetylates H4 histone molecules in H3.1-H4 over H3.3-H4. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 9. pp. 6573-6581.
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