Human histamine N-methyltransferase gene

Structural characterization and chromosomal localization

Saime Aksoy, Rebecca Raftogianis, Richard M Weinshilboum

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Histamine N-methyltransferase (HNMT) catalyzes the N(τ)-methylation of histamine. The level of HNMT activity in human red blood cells is controlled by a common genetic polymorphism. We previously cloned and expressed a cDNA for human kidney HNMT, and we have now determined the structural organization of the human HNMT gene as a step toward studies of the genetic regulation of levels of HNMT activity in human tissue. Structural characterization of the HNMT gene was performed by use of a polymerase chain reaction (PCR)-based strategy. The gene was approximately 34 kb in length and contained 6 exons. All exon-intron splice junction sequences conformed to the 'GT-AG' rule. The longest transcript isolated after 5'-rapid amplification of cDNA ends indicated that transcription initiation occurred 252 nucleotides 5'-upstream from the cDNA translation initiation codon. HNMT mapped to human chromosome 2. Structural characterization of the gene for HNMT will make it possible to study molecular generic mechanisms involved in the regulation of this important enzyme in humans.

Original languageEnglish (US)
Pages (from-to)548-554
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume219
Issue number2
DOIs
StatePublished - Feb 15 1996

Fingerprint

Histamine N-Methyltransferase
Genes
Complementary DNA
Human Activities
Exons
Methylation
Chromosomes, Human, Pair 2
Initiator Codon
Polymerase chain reaction
Human Chromosomes
Genetic Polymorphisms
Transcription
Chromosomes
Polymorphism
Introns
Histamine
Amplification
Blood
Nucleotides
Erythrocytes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Human histamine N-methyltransferase gene : Structural characterization and chromosomal localization. / Aksoy, Saime; Raftogianis, Rebecca; Weinshilboum, Richard M.

In: Biochemical and Biophysical Research Communications, Vol. 219, No. 2, 15.02.1996, p. 548-554.

Research output: Contribution to journalArticle

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