TY - JOUR
T1 - Human glucocorticoid receptor α gene (NR3C1) pharmacogenomics
T2 - Gene resequencing and functional genomics
AU - Niu, Nifang
AU - Manickam, Venkatraman
AU - Kalari, Krishna R.
AU - Moon, Irene
AU - Pelleymounter, Linda L.
AU - Eckloff, Bruce W.
AU - Wieben, Eric D.
AU - Schaid, Daniel J.
AU - Wang, Liewei
PY - 2009/8
Y1 - 2009/8
N2 - Context: The human glucocorticoid receptor α (GRα) is a nuclear hormone receptor that regulates multiple physiological and pathophysiological processes. There are large variations in both physiological and therapeutic response to glucocorticoids. Multiple previous studies suggested that genetic polymorphisms in GRα (NR3C1) might play an important role. Objective: The aim of the study was to identify and determine the functional implications of common genetic variation in NR3C1. Design: We resequenced the NR3C1 gene using 240 DNA samples from four ethnic groups, followed by functional characterization of the effects of selected polymorphisms. Results: A total of 108 polymorphisms were identified in GRα, including nine nonsynonymous coding single nucleotide polymorphisms (cSNPs) and four synonymous cSNPs with a minor allele frequency greater than 5%. Functional studies showed that SNPs encoding Phe(65)Val and Asp(687)Glu displayed slightly increased levels of protein compared with WT, and Asp(687)Glu also caused increased GRα receptor number. In addition, Ala(229)Thr and Ile(292)Val showed slightly decreased ligand binding affinity in COS-1 cells. A genotype-phenotype association study of NR3C1 gene expression in 240 lymphoblastoid cell lines identified one SNP, Cm746T>C, located 5′-upstream of noncoding exon 1C, and one haplotype, Cm237delC/Cm238C>T/Cm240G>C in exon 1C of the gene that were associated with GRα mRNA expression and a trend with GRα number. Conclusions: These results represent a step toward understanding the functional role of common sequence variation in the GRα gene (NR3C1) and the potential application of those SNPs in translational studies.
AB - Context: The human glucocorticoid receptor α (GRα) is a nuclear hormone receptor that regulates multiple physiological and pathophysiological processes. There are large variations in both physiological and therapeutic response to glucocorticoids. Multiple previous studies suggested that genetic polymorphisms in GRα (NR3C1) might play an important role. Objective: The aim of the study was to identify and determine the functional implications of common genetic variation in NR3C1. Design: We resequenced the NR3C1 gene using 240 DNA samples from four ethnic groups, followed by functional characterization of the effects of selected polymorphisms. Results: A total of 108 polymorphisms were identified in GRα, including nine nonsynonymous coding single nucleotide polymorphisms (cSNPs) and four synonymous cSNPs with a minor allele frequency greater than 5%. Functional studies showed that SNPs encoding Phe(65)Val and Asp(687)Glu displayed slightly increased levels of protein compared with WT, and Asp(687)Glu also caused increased GRα receptor number. In addition, Ala(229)Thr and Ile(292)Val showed slightly decreased ligand binding affinity in COS-1 cells. A genotype-phenotype association study of NR3C1 gene expression in 240 lymphoblastoid cell lines identified one SNP, Cm746T>C, located 5′-upstream of noncoding exon 1C, and one haplotype, Cm237delC/Cm238C>T/Cm240G>C in exon 1C of the gene that were associated with GRα mRNA expression and a trend with GRα number. Conclusions: These results represent a step toward understanding the functional role of common sequence variation in the GRα gene (NR3C1) and the potential application of those SNPs in translational studies.
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U2 - 10.1210/jc.2008-2109
DO - 10.1210/jc.2008-2109
M3 - Article
C2 - 19435830
AN - SCOPUS:68549088756
SN - 0021-972X
VL - 94
SP - 3072
EP - 3084
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
IS - 8
ER -