Human finger-prick induced pluripotent stem cells facilitate the development of stem cell banking

Hong Keetan Tan, Cheng Xu Delon Toh, Dongrui Ma, Binxia Yang, Tong Ming Liu, Jun Lu, Chee Wai Wong, Tze Kai Tan, Hu Li, Christopher Syn, Eng Lee Tan, Bing Lim, Yoon Pin Lim, Stuart A. Cook, Yuin Han Loh

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Induced pluripotent stem cells (iPSCs) derived from somatic cells of patients can be a good model for studying human diseases and for future therapeutic regenerative medicine. Current initiatives to establish human iPSC (hiPSC) banking face challenges in recruiting large numbers of donors with diverse diseased, genetic, and phenotypic representations. In this study, we describe the efficient derivation of transgene-free hiPSCs from human finger-prick blood. Finger-prick sample collection can be performed on a "do-it-yourself" basis by donors and sent to the hiPSC facility for reprogramming. We show that single-drop volumes of finger-prick samples are sufficient for performing cellular reprogramming, DNA sequencing, and blood serotyping in parallel. Our novel strategy has the potential to facilitate the development of large-scale hiPSC banking worldwide.

Original languageEnglish (US)
Pages (from-to)586-598
Number of pages13
JournalStem Cells Translational Medicine
Volume3
Issue number5
DOIs
StatePublished - 2014

Keywords

  • Finger prick
  • Human induced pluripotent stem cell banking
  • Human peripheral blood
  • Induced pluripotent stem cells
  • Reprogramming

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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