Human embryonic stem cells are pre-mitotically committed to self-renewal and acquire a lengthened G1 phase upon lineage programming

Klaus A. Becker, Janet L. Stein, Jane B. Lian, Andre J van Wijnen, Gary S. Stein

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Self-renewal of human embryonic stem (hES) cells proceeds by a unique abbreviated cell cycle with a shortened G1 phase and distinctions in molecular cell cycle regulatory parameters. In this study, we show that early lineage-commitment of pluripotent hES cells modifies cell cycle kinetics. Human ES cells acquire a lengthened G1 within 72 h after lineage-programming is initiated, as reflected by loss of the pluripotency factor Oct4 and alterations in nuclear morphology. In hES cells that maintain the pristine pluripotent state, we find that autocrine mechanisms contribute to sustaining the abbreviated cell cycle. Our data show that naïve and mitotically synchronized pluripotent hES cells are competent to initiate two consecutive S phases in the absence of external growth factors. We conclude that short-term self-renewal of pluripotent hES cells occurs autonomously, in part due to secreted factors, and that pluripotency is functionally linked to the abbreviated hES cell cycle.

Original languageEnglish (US)
Pages (from-to)103-110
Number of pages8
JournalJournal of Cellular Physiology
Volume222
Issue number1
DOIs
StatePublished - Jan 2010
Externally publishedYes

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G1 Phase
Stem cells
Cell Cycle
Cells
S Phase
Human Embryonic Stem Cells
Intercellular Signaling Peptides and Proteins
Kinetics

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Human embryonic stem cells are pre-mitotically committed to self-renewal and acquire a lengthened G1 phase upon lineage programming. / Becker, Klaus A.; Stein, Janet L.; Lian, Jane B.; van Wijnen, Andre J; Stein, Gary S.

In: Journal of Cellular Physiology, Vol. 222, No. 1, 01.2010, p. 103-110.

Research output: Contribution to journalArticle

Becker, Klaus A. ; Stein, Janet L. ; Lian, Jane B. ; van Wijnen, Andre J ; Stein, Gary S. / Human embryonic stem cells are pre-mitotically committed to self-renewal and acquire a lengthened G1 phase upon lineage programming. In: Journal of Cellular Physiology. 2010 ; Vol. 222, No. 1. pp. 103-110.
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