Abstract
Mesenchymal stem/stromal cells (MSCs) have been isolated from various tissues and utilized for an expanding number of therapies. The developmental pathways involved in producing MSCs and the phenotypic precursor/progenitor cells that give rise to human MSCs remain poorly defined. Human embryonic stem cells (hESCs) have the capability to generate functional hemato-endothelial cells and other mesoderm lineage cells. hESC-derived CD73+ cells have been isolated and found to have similar phenotypic and functional characteristics as adult MSCs. Here we demonstrate hESC-derived CD34+CD73- cells can serve as MSC progenitor cells with the ability to differentiate into adipocytes, osteoblasts and chondrocytes. Additionally, gene array analysis of hESC-derived MSCs show substantially different gene expression compared to bone marrow (BM)-derived MSCs, especially with increased expression of pluripotent and multipotent stem cell and endothelial cell-associated genes. The isolation of functional MSCs from hESC-derived CD34+CD73- cells provides improved understanding of MSC development and utilization of pluripotent stem cells to produce MSCs suited for novel regenerative therapies.
Original language | English (US) |
---|---|
Pages (from-to) | 718-728 |
Number of pages | 11 |
Journal | Bone |
Volume | 47 |
Issue number | 4 |
DOIs | |
State | Published - Oct 2010 |
Keywords
- Differentiation
- Hematopoietic stem cells
- Human embryonic stem cells
- Mesenchymal stem cells
- Progenitor cells
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Physiology
- Histology