Human embryonic stem cell-derived CD34+ cells function as MSC progenitor cells

Ross A. Kopher, Vesselin R. Penchev, Mohammad S. Islam, Katherine L. Hill, Sundeep Khosla, Dan S. Kaufman

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

Mesenchymal stem/stromal cells (MSCs) have been isolated from various tissues and utilized for an expanding number of therapies. The developmental pathways involved in producing MSCs and the phenotypic precursor/progenitor cells that give rise to human MSCs remain poorly defined. Human embryonic stem cells (hESCs) have the capability to generate functional hemato-endothelial cells and other mesoderm lineage cells. hESC-derived CD73+ cells have been isolated and found to have similar phenotypic and functional characteristics as adult MSCs. Here we demonstrate hESC-derived CD34+CD73- cells can serve as MSC progenitor cells with the ability to differentiate into adipocytes, osteoblasts and chondrocytes. Additionally, gene array analysis of hESC-derived MSCs show substantially different gene expression compared to bone marrow (BM)-derived MSCs, especially with increased expression of pluripotent and multipotent stem cell and endothelial cell-associated genes. The isolation of functional MSCs from hESC-derived CD34+CD73- cells provides improved understanding of MSC development and utilization of pluripotent stem cells to produce MSCs suited for novel regenerative therapies.

Original languageEnglish (US)
Pages (from-to)718-728
Number of pages11
JournalBone
Volume47
Issue number4
DOIs
StatePublished - Oct 1 2010

Keywords

  • Differentiation
  • Hematopoietic stem cells
  • Human embryonic stem cells
  • Mesenchymal stem cells
  • Progenitor cells

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Histology

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    Kopher, R. A., Penchev, V. R., Islam, M. S., Hill, K. L., Khosla, S., & Kaufman, D. S. (2010). Human embryonic stem cell-derived CD34+ cells function as MSC progenitor cells. Bone, 47(4), 718-728. https://doi.org/10.1016/j.bone.2010.06.020