Human cytosolic sulfotransferase database mining

Identification of seven novel genes and pseudogenes

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

A total of 10 SULT genes are presently known to be expressed in human tissues. We performed a comprehensive genome-wide search for novel SULT genes using two different but complementary approaches, and developed a novel graphical display to aid in the annotation of the hits. Seven novel human SULT genes were identified, five of which were predicted to be pseudogenes, including two processed pseudogenes and three pseudogenes that contained introns. Those five pseudogenes represent the first unambiguous SULT pseudogenes described in any species. Expression-profiling studies were conducted for one novel gene, SULT6B1, and a series of alternatively spliced transcripts were identified in the human testis. SULT6B1 was also present in chimpanzee and gorilla, differing at only seven encoded amino-acid residues among the three species. The results of these database mining studies will aid in studies of the regulation of these SULT genes, provide insights into the evolution of this gene family in humans, and serve as a starting point for comparative genomic studies of SULT genes.

Original languageEnglish (US)
Pages (from-to)54-65
Number of pages12
JournalPharmacogenomics Journal
Volume4
Issue number1
DOIs
StatePublished - 2004

Fingerprint

Sulfotransferases
Pseudogenes
Databases
Genes
Gorilla gorilla
Pan troglodytes
Introns
Testis
Genome
Amino Acids

Keywords

  • Annotation
  • Database mining
  • Gene
  • Pseudogene
  • Sulfation
  • Sulfotransferase
  • SULT

ASJC Scopus subject areas

  • Pharmacology
  • Molecular Medicine
  • Genetics

Cite this

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title = "Human cytosolic sulfotransferase database mining: Identification of seven novel genes and pseudogenes",
abstract = "A total of 10 SULT genes are presently known to be expressed in human tissues. We performed a comprehensive genome-wide search for novel SULT genes using two different but complementary approaches, and developed a novel graphical display to aid in the annotation of the hits. Seven novel human SULT genes were identified, five of which were predicted to be pseudogenes, including two processed pseudogenes and three pseudogenes that contained introns. Those five pseudogenes represent the first unambiguous SULT pseudogenes described in any species. Expression-profiling studies were conducted for one novel gene, SULT6B1, and a series of alternatively spliced transcripts were identified in the human testis. SULT6B1 was also present in chimpanzee and gorilla, differing at only seven encoded amino-acid residues among the three species. The results of these database mining studies will aid in studies of the regulation of these SULT genes, provide insights into the evolution of this gene family in humans, and serve as a starting point for comparative genomic studies of SULT genes.",
keywords = "Annotation, Database mining, Gene, Pseudogene, Sulfation, Sulfotransferase, SULT",
author = "Robert Freimuth and M. Wiepert and Chute, {C. G.} and Wieben, {Eric D} and Weinshilboum, {Richard M}",
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AU - Chute, C. G.

AU - Wieben, Eric D

AU - Weinshilboum, Richard M

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N2 - A total of 10 SULT genes are presently known to be expressed in human tissues. We performed a comprehensive genome-wide search for novel SULT genes using two different but complementary approaches, and developed a novel graphical display to aid in the annotation of the hits. Seven novel human SULT genes were identified, five of which were predicted to be pseudogenes, including two processed pseudogenes and three pseudogenes that contained introns. Those five pseudogenes represent the first unambiguous SULT pseudogenes described in any species. Expression-profiling studies were conducted for one novel gene, SULT6B1, and a series of alternatively spliced transcripts were identified in the human testis. SULT6B1 was also present in chimpanzee and gorilla, differing at only seven encoded amino-acid residues among the three species. The results of these database mining studies will aid in studies of the regulation of these SULT genes, provide insights into the evolution of this gene family in humans, and serve as a starting point for comparative genomic studies of SULT genes.

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KW - Annotation

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KW - Pseudogene

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KW - Sulfotransferase

KW - SULT

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