Human cerebrospinal fluid regulates proliferation and migration of stem cells through insulin-like growth factor-1

Mingxin Zhu, Yun Feng, Sean Dangelmajer, Hugo Guerrero Cazares, Kaisorn L. Chaichana, Christopher L. Smith, Andre Levchenko, Ting Lei, Alfredo Quinones-Hinojosa

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSCs) and neural progenitor cells (NPCs) have been regarded for their clinical therapeutic potential for central nervous system (CNS) pathologies. Their potential utility is a result of their intrinsic ability to repair damaged tissues, deliver therapeutic proteins, and migrate to sites of pathology within the brain. However, it remains unclear whether the CNS promotes any changes in these potential therapeutic cells, which would be critical to understand before clinical application. A major component of the CNS is cerebrospinal fluid (CSF). Therefore, the aim of this study was to evaluate the influence that human CSF has on the function of human adipose-derived MSCs (hAMSCs) and human fetal-derived NPCs (hfNPCs) in regard to cell proliferation, survival, and migration. This study demonstrated that human noncancerous CSF promoted proliferation and inhibited apoptosis of hAMSCs and hfNPCs. Preculturing these stem cells in human CSF also increased their migratory speed and distance traveled. Furthermore, insulin-like growth factor-1 (IGF-1) in human CSF enhanced the migration capacity and increased the expression of C-X-C chemokine receptor type 4 (CXCR4) in both stem cell types. These current findings highlight a simple and natural way in which human CSF can enhance the proliferation, migration, and viability of human exogenous primary hAMSCs and hfNPCs. This study may provide insight into improving the clinical efficacy of stem cells for the treatment of CNS pathologies.

Original languageEnglish (US)
Pages (from-to)160-171
Number of pages12
JournalStem Cells and Development
Volume24
Issue number2
DOIs
StatePublished - Jan 15 2015
Externally publishedYes

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Somatomedins
Cerebrospinal Fluid
Stem Cells
Central Nervous System
Pathology
Mesenchymal Stromal Cells
CXC Chemokines
Chemokine Receptors
Cell Movement
Cell Survival
Therapeutics
Cell Proliferation
Apoptosis

ASJC Scopus subject areas

  • Hematology
  • Developmental Biology
  • Cell Biology

Cite this

Human cerebrospinal fluid regulates proliferation and migration of stem cells through insulin-like growth factor-1. / Zhu, Mingxin; Feng, Yun; Dangelmajer, Sean; Guerrero Cazares, Hugo; Chaichana, Kaisorn L.; Smith, Christopher L.; Levchenko, Andre; Lei, Ting; Quinones-Hinojosa, Alfredo.

In: Stem Cells and Development, Vol. 24, No. 2, 15.01.2015, p. 160-171.

Research output: Contribution to journalArticle

Zhu, Mingxin ; Feng, Yun ; Dangelmajer, Sean ; Guerrero Cazares, Hugo ; Chaichana, Kaisorn L. ; Smith, Christopher L. ; Levchenko, Andre ; Lei, Ting ; Quinones-Hinojosa, Alfredo. / Human cerebrospinal fluid regulates proliferation and migration of stem cells through insulin-like growth factor-1. In: Stem Cells and Development. 2015 ; Vol. 24, No. 2. pp. 160-171.
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