Human and rat osteoblast-like cells express stathmin, a growth-regulatory protein

Rajiv Kumar, J. D. Haugen

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

We used the differential display polymerase chain reaction method of Liang and Pardee (Science (1992) 257:967-971) to identify a growth-regulatory protein, stathmin or oncoprotein 18, in human and rat osteoblast-like cells. When messenger ribonucleic acid from human osteosarcoma (Saos-2) cells treated with 10-8 1,25-dihydroxyvitamin D3 was compared with that of cells treated with vehicle alone by this method, we observed an increase in the intensity of a band that on subsequent DNA sequence analysis was found to encode stathmin. We examined normal human osteoblast-like cells, transformed human osteoblast-like cells (HOBIT) human osteosarcoma cells (Saos-2), and rat osteoblast cell (ROS 17/2.8, ROS 25/1) lines for the presence of the messenger RNA for stathmin. All osteoblast-like cells examined expressed the RNA for this protein. Western analysis of the protein of these cells with a polyclonal antibody directed against stathmin showed the presence of a M(r) 19,000 band. We conclude that human and rat osteoblast-like cells express stathmin and that this protein could play a role in regulation of the growth of these cells in response to various hormonal stimuli.

Original languageEnglish (US)
Pages (from-to)861-865
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume201
Issue number2
DOIs
StatePublished - 1994

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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