Human and mouse eosinophils have antiviral activity against parainfluenza virus

Matthew G. Drake, Elizabeth R. Bivins-Smith, Becky J. Proskocil, Zhenying Nie, Gregory D. Scott, James J. Lee, Nancy A Lee, Allison D. Fryer, David B. Jacoby

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Respiratory viruses cause asthma exacerbations. Because eosinophils are the prominent leukocytes in the airways of 60-70% of patients with asthma, we evaluated the effects of eosinophils on a common respiratory virus, parainfluenza 1, in the lung. Eosinophils recruited to the airways of wild-type mice after ovalbumin sensitization and challenge significantly decreased parainfluenza virus RNA in the lungs 4 days after infection compared with nonsensitized animals. This antiviral effect was also seen in IL-5 transgenic mice with an abundance of airway eosinophils (NJ.1726) but was lost in transgenic eosinophil-deficient mice (PHIL) and in IL-5 transgenic mice crossed with eosinophil-deficient mice (NJ.1726-PHIL). Loss of the eosinophil granule protein eosinophil peroxidase, using eosinophil peroxidase-deficient transgenic mice, did not reduce eosinophils' antiviral effect. Eosinophil antiviral mechanisms were also explored in vitro. Isolated human eosinophils significantly reduced parainfluenza virus titers. This effect did not involve degradation of viral RNA by eosinophil granule RNases. However, eosinophils treated with a nitric oxide synthase inhibitor lost their antiviral activity, suggesting eosinophils attenuate viral infectivity through production of nitric oxide. Consequently, eosinophil nitric oxide production was measured with an intracellular fluorescent probe. Eosinophils produced nitric oxide in response to virus and to a synthetic agonist of the virus-sensing innate immune receptor, Toll-like receptor (TLR) 7. IFNg increased expression of eosinophil TLR7 and potentiated TLR7-induced nitric oxide production. These results suggest that eosinophils promote viral clearance in the lung and contribute to innate immune responses against respiratory virus infections in humans.

Original languageEnglish (US)
Pages (from-to)387-394
Number of pages8
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume55
Issue number3
DOIs
StatePublished - Sep 1 2016

Fingerprint

Paramyxoviridae Infections
Viruses
Eosinophils
Antiviral Agents
Nitric Oxide
Eosinophil Peroxidase
Interleukin-5
Eosinophil Granule Proteins
Toll-Like Receptor 7
Transgenic Mice
Ovalbumin
Viral RNA
Ribonucleases
Fluorescent Dyes
Nitric Oxide Synthase
Lung
Animals
RNA
Asthma
Degradation

Keywords

  • Asthma
  • Eosinophil
  • Nitric oxide
  • Parainfluenza virus
  • Toll-like receptor 7

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Drake, M. G., Bivins-Smith, E. R., Proskocil, B. J., Nie, Z., Scott, G. D., Lee, J. J., ... Jacoby, D. B. (2016). Human and mouse eosinophils have antiviral activity against parainfluenza virus. American Journal of Respiratory Cell and Molecular Biology, 55(3), 387-394. https://doi.org/10.1165/rcmb.2015-0405OC

Human and mouse eosinophils have antiviral activity against parainfluenza virus. / Drake, Matthew G.; Bivins-Smith, Elizabeth R.; Proskocil, Becky J.; Nie, Zhenying; Scott, Gregory D.; Lee, James J.; Lee, Nancy A; Fryer, Allison D.; Jacoby, David B.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 55, No. 3, 01.09.2016, p. 387-394.

Research output: Contribution to journalArticle

Drake, MG, Bivins-Smith, ER, Proskocil, BJ, Nie, Z, Scott, GD, Lee, JJ, Lee, NA, Fryer, AD & Jacoby, DB 2016, 'Human and mouse eosinophils have antiviral activity against parainfluenza virus', American Journal of Respiratory Cell and Molecular Biology, vol. 55, no. 3, pp. 387-394. https://doi.org/10.1165/rcmb.2015-0405OC
Drake, Matthew G. ; Bivins-Smith, Elizabeth R. ; Proskocil, Becky J. ; Nie, Zhenying ; Scott, Gregory D. ; Lee, James J. ; Lee, Nancy A ; Fryer, Allison D. ; Jacoby, David B. / Human and mouse eosinophils have antiviral activity against parainfluenza virus. In: American Journal of Respiratory Cell and Molecular Biology. 2016 ; Vol. 55, No. 3. pp. 387-394.
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