Human Adipose-Derived Mesenchymal Stromal/Stem Cells Remain Viable and Metabolically Active Following Needle Passage

Kentaro Onishi, Dakota L. Jones, Scott M. Riester, Eric A. Lewallen, David G. Lewallen, Jacob L. Sellon, Allan B Dietz, Wenchun Qu, Andre J van Wijnen, Jay Smith

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective: To assess the biological effects of passage through clinically relevant needles on the viability and metabolic activity of culture-expanded, human adipose tissue-derived mesenchymal stromal/stem cells (AMSCs). Design: Prospective observational pilot study. Setting: Academic medical center. Participants: Patient-derived clinical-grade culture expanded AMSCs. Interventions: AMSCs were passed through syringes without a needle attached (control), with an 18-gauge (25.4-mm) needle attached and with a 30-gauge (19-mm) needle attached at a constant injection flow rate and constant cell concentrations. Each injection condition was completed in triplicate. Main Outcome Measures: Cell number and viability, proliferative capacity, metabolic activity, and acute gene expression as measured by cell counts, mitochondrial activity, and quantitative real time reverse-transcription polymerase chain reaction on day 0 (immediately), day 1, and day 4 after injection. Results: AMSC viability was not significantly affected by injection, and cells proliferated normally regardless of study group. Postinjection, AMSCs robustly expressed both proliferation markers and extracellular matrix proteins. Stress-response mRNAs were markedly but transiently increased independently of needle size within the first day in culture postinjection. Conclusions: Human, culture-expanded AMSCs maintain their viability, proliferative capacity, and metabolic function following passage through needles as small as 30-gauge at constant flow rates of 4 mL/min, despite an early, nonspecific stress/cytoprotective response. These initial findings suggest that culture-expanded AMSCs should tolerate the injection process during most cell-based therapeutic interventions.

Original languageEnglish (US)
JournalPM and R
DOIs
StateAccepted/In press - Sep 29 2015

Fingerprint

Mesenchymal Stromal Cells
Needles
Adipose Tissue
Injections
Cell Survival
Cell Count
Extracellular Matrix Proteins
Syringes
Reverse Transcription
Observational Studies
Outcome Assessment (Health Care)

ASJC Scopus subject areas

  • Rehabilitation
  • Neurology
  • Clinical Neurology
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Human Adipose-Derived Mesenchymal Stromal/Stem Cells Remain Viable and Metabolically Active Following Needle Passage. / Onishi, Kentaro; Jones, Dakota L.; Riester, Scott M.; Lewallen, Eric A.; Lewallen, David G.; Sellon, Jacob L.; Dietz, Allan B; Qu, Wenchun; van Wijnen, Andre J; Smith, Jay.

In: PM and R, 29.09.2015.

Research output: Contribution to journalArticle

Onishi, Kentaro ; Jones, Dakota L. ; Riester, Scott M. ; Lewallen, Eric A. ; Lewallen, David G. ; Sellon, Jacob L. ; Dietz, Allan B ; Qu, Wenchun ; van Wijnen, Andre J ; Smith, Jay. / Human Adipose-Derived Mesenchymal Stromal/Stem Cells Remain Viable and Metabolically Active Following Needle Passage. In: PM and R. 2015.
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abstract = "Objective: To assess the biological effects of passage through clinically relevant needles on the viability and metabolic activity of culture-expanded, human adipose tissue-derived mesenchymal stromal/stem cells (AMSCs). Design: Prospective observational pilot study. Setting: Academic medical center. Participants: Patient-derived clinical-grade culture expanded AMSCs. Interventions: AMSCs were passed through syringes without a needle attached (control), with an 18-gauge (25.4-mm) needle attached and with a 30-gauge (19-mm) needle attached at a constant injection flow rate and constant cell concentrations. Each injection condition was completed in triplicate. Main Outcome Measures: Cell number and viability, proliferative capacity, metabolic activity, and acute gene expression as measured by cell counts, mitochondrial activity, and quantitative real time reverse-transcription polymerase chain reaction on day 0 (immediately), day 1, and day 4 after injection. Results: AMSC viability was not significantly affected by injection, and cells proliferated normally regardless of study group. Postinjection, AMSCs robustly expressed both proliferation markers and extracellular matrix proteins. Stress-response mRNAs were markedly but transiently increased independently of needle size within the first day in culture postinjection. Conclusions: Human, culture-expanded AMSCs maintain their viability, proliferative capacity, and metabolic function following passage through needles as small as 30-gauge at constant flow rates of 4 mL/min, despite an early, nonspecific stress/cytoprotective response. These initial findings suggest that culture-expanded AMSCs should tolerate the injection process during most cell-based therapeutic interventions.",
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AU - Lewallen, Eric A.

AU - Lewallen, David G.

AU - Sellon, Jacob L.

AU - Dietz, Allan B

AU - Qu, Wenchun

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