Human κ chain expression in a λ phage vector: methods of isolating amplified cDNA affects cloning efficiency

Konstantinos Lazaridis, Howard Dang, Norman Talal

Research output: Contribution to journalArticle

Abstract

Three common methods of isolating amplified DNA were evaluated for their effects on cloning efficiency in a phage vector designed to express human κ chain. The “glass milk” technique gave higher cloning efficiency and protein expression than phenol-chloroform extraction or microfiltration. This shows that the quality of amplified cDNA should be considered when studying the human antibody repertoire using this vector system.

Original languageEnglish (US)
Pages (from-to)104-106
Number of pages3
JournalHuman Antibodies
Volume4
Issue number3
DOIs
StatePublished - Jan 1 1993

Keywords

  • Dna isolation
  • Polymerase chain reaction
  • Recombinant antibody

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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