TY - JOUR
T1 - Human κ chain expression in a λ phage vector
T2 - methods of isolating amplified cDNA affects cloning efficiency
AU - Lazaridis, Konstantinos
AU - Dang, Howard
AU - Talal, Norman
PY - 1993
Y1 - 1993
N2 - Three common methods of isolating amplified DNA were evaluated for their effects on cloning efficiency in a phage vector designed to express human κ chain. The “glass milk” technique gave higher cloning efficiency and protein expression than phenol-chloroform extraction or microfiltration. This shows that the quality of amplified cDNA should be considered when studying the human antibody repertoire using this vector system.
AB - Three common methods of isolating amplified DNA were evaluated for their effects on cloning efficiency in a phage vector designed to express human κ chain. The “glass milk” technique gave higher cloning efficiency and protein expression than phenol-chloroform extraction or microfiltration. This shows that the quality of amplified cDNA should be considered when studying the human antibody repertoire using this vector system.
KW - Dna isolation
KW - Polymerase chain reaction
KW - Recombinant antibody
UR - http://www.scopus.com/inward/record.url?scp=0027327110&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027327110&partnerID=8YFLogxK
U2 - 10.3233/HAB-1993-4302
DO - 10.3233/HAB-1993-4302
M3 - Article
C2 - 8357956
AN - SCOPUS:0027327110
SN - 1093-2607
VL - 4
SP - 104
EP - 106
JO - Human Antibodies
JF - Human Antibodies
IS - 3
ER -