Abstract
According to the hypothesis that most glucose absorption occurs passively across intestinal tight junctions (paracellular absorption), one would predict fairly similar rates of in vivo absorption of L-glucose, the stereoisomer of D-glucose that is absorbed only passively and is not catabolized, and of 3-O-methyl-D-glucose (3OMD-glucose), the D-glucose analogue that is actively and passively transported and not catabolized. In house sparrows Passer domesticus, we applied a pharmacokinetic method to measure simultaneous in vivo absorption of [14C] L-glucose and [3H]3OMD-glucose in a situation in which intestinal glucose transporters were relatively saturated (gavage solution contained 200 mmol l-1 3OMD-glucose). Fractional absorptions (F) were not significantly different between [3H]3OMD- and [ 14C]L-glucose (0.80 vs 0.79), and the apparent rates of absorption did not differ significantly. When we performed the same experiment on other sparrows in a situation in which intestinal glucose transporters were relatively unsaturated (200 mmol l-1 mannitol replaced 3OMD-glucose in the gavage solution), the apparent rate of absorption was significantly reduced for [14C]L-glucose by 39% and for [3H]3OMD-glucose by 26%. A simulation model showed that a reduction is not predicted if most of the [ 3H]3OMD-glucose is actively absorbed, because the absorption rate of the tracer should increase when competitive inhibitor (unlabeled 3OMD-glucose) is removed. The similar extent and rates of absorption of [3H]3OMD- and [14C]L-glucose, and the acceleration of their rates of absorption in the presence of luminal 3OMD-glucose, are most consistent with Pappenheimer's hypothesis that the majority of dietary D-glucose is absorbed passively.
Original language | English (US) |
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Pages (from-to) | 3109-3121 |
Number of pages | 13 |
Journal | Journal of Experimental Biology |
Volume | 207 |
Issue number | 17 |
DOIs | |
State | Published - Aug 2004 |
Keywords
- 3-O-methyl-D-glucose mediated absorption
- D-glucose
- House sparrow
- Passer domesticus
- Passive absorption
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Physiology
- Aquatic Science
- Animal Science and Zoology
- Molecular Biology
- Insect Science