How myosin VI coordinates its heads during processive movement

H. Lee Sweeney, Hyokeun Park, Alan B. Zong, Zhaohui Yang, Paul R. Selvin, Steven Rosenfeld

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

A processive molecular motor must coordinate the enzymatic state of its two catalytic domains in order to prevent premature detachment from its track. For myosin V, internal strain produced when both heads of are attached to an actin track prevents completion of the lever arm swing of the lead head and blocks ADP release. However, this mechanism cannot work for myosin VI, since its lever arm positions are reversed. Here, we demonstrate that myosin VI gating is achieved instead by blocking ATP binding to the lead head once it has released its ADP. The structural basis for this unique gating mechanism involves an insert near the nucleotide binding pocket that is found only in class VI myosin. Reverse strain greatly favors binding of ADP to the lead head, which makes it possible for myosin VI to function as a processive transporter as well as an actin-based anchor. While this mechanism is unlike that of any other myosin superfamily member, it bears remarkable similarities to that of another processive motor from a different superfamily - kinesin I.

Original languageEnglish (US)
Pages (from-to)2682-2692
Number of pages11
JournalEMBO Journal
Volume26
Issue number11
DOIs
StatePublished - Jun 6 2007
Externally publishedYes

Fingerprint

Head
Adenosine Diphosphate
Actins
Myosin Type V
Kinesin
Myosins
Anchors
Catalytic Domain
Nucleotides
Adenosine Triphosphate
myosin VI
Lead

Keywords

  • Gating
  • Kinetics
  • Motility
  • Processivity
  • Unconventional myosin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Sweeney, H. L., Park, H., Zong, A. B., Yang, Z., Selvin, P. R., & Rosenfeld, S. (2007). How myosin VI coordinates its heads during processive movement. EMBO Journal, 26(11), 2682-2692. https://doi.org/10.1038/sj.emboj.7601720

How myosin VI coordinates its heads during processive movement. / Sweeney, H. Lee; Park, Hyokeun; Zong, Alan B.; Yang, Zhaohui; Selvin, Paul R.; Rosenfeld, Steven.

In: EMBO Journal, Vol. 26, No. 11, 06.06.2007, p. 2682-2692.

Research output: Contribution to journalArticle

Sweeney, HL, Park, H, Zong, AB, Yang, Z, Selvin, PR & Rosenfeld, S 2007, 'How myosin VI coordinates its heads during processive movement', EMBO Journal, vol. 26, no. 11, pp. 2682-2692. https://doi.org/10.1038/sj.emboj.7601720
Sweeney, H. Lee ; Park, Hyokeun ; Zong, Alan B. ; Yang, Zhaohui ; Selvin, Paul R. ; Rosenfeld, Steven. / How myosin VI coordinates its heads during processive movement. In: EMBO Journal. 2007 ; Vol. 26, No. 11. pp. 2682-2692.
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