Host-derived angiogenesis maintains bone blood flow after withdrawal of immunosuppression

Teruyasu Ohno, Michael Pelzer, Mikko Larsen, Patricia F. Friedrich, Allen Thorp Bishop

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A novel method of living bone allotransplantation combining microvascular repair of the nutrient circulation, implantation of host-derived arteriovenous (AV) bundles, and short-term immunosuppression is described. We hypothesized that neoangiogenesis from the implanted vessels would maintain graft viability and circulation after withdrawal of FK506 (Tacrolimus) immunosuppression. Vascularized femoral transplantation was performed between DA and PVG rats. In addition to microsurgical pedicle anastomoses, a saphenous AV bundle from the recipient animal was implanted in the medullary space. Ninety-seven rats were randomly allocated to groups differing in immunosuppression and AV bundle patency. Implanted vessels significantly improved capillary density and bone blood flow in nonimmunosuppressed and immmunosuppressed groups, respectively. A lower incidence of spontaneous AV bundle thrombosis was found with Tacrolimus treatment. More viable osteocytes were seen at 4 weeks when the AV bundle was patent. Further investigations may confirm host-derived neoangiogenesis as an alternative to tolerance induction or immunosuppression in bone allotransplantation.

Original languageEnglish (US)
Pages (from-to)657-663
Number of pages7
JournalMicrosurgery
Volume27
Issue number8
DOIs
StatePublished - 2007

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Immunosuppression
Tacrolimus
Bone and Bones
Osteocytes
Thigh
Bone Density
Thrombosis
Transplantation
Transplants
Food
Incidence
Therapeutics

ASJC Scopus subject areas

  • Surgery

Cite this

Host-derived angiogenesis maintains bone blood flow after withdrawal of immunosuppression. / Ohno, Teruyasu; Pelzer, Michael; Larsen, Mikko; Friedrich, Patricia F.; Bishop, Allen Thorp.

In: Microsurgery, Vol. 27, No. 8, 2007, p. 657-663.

Research output: Contribution to journalArticle

Ohno, Teruyasu ; Pelzer, Michael ; Larsen, Mikko ; Friedrich, Patricia F. ; Bishop, Allen Thorp. / Host-derived angiogenesis maintains bone blood flow after withdrawal of immunosuppression. In: Microsurgery. 2007 ; Vol. 27, No. 8. pp. 657-663.
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