TY - JOUR
T1 - Hormonal regulation of transcription of rDNA
T2 - Glucocorticoid effects upon initiation and elongation in vitro
AU - Cavanaugh, Alice H.
AU - Thompson, E. Aubrey
N1 - Funding Information:
This work was supported by NIH grant CA22394.
PY - 1985/5/10
Y1 - 1985/5/10
N2 - Various parameters of transcription of cloned mouse rDNA have been examined using extracts from control P1798 cells and from cells treated 24h with 0.1 μM dexamethasone. Highly purified RNA polymerase I from either source catalyzes nucleotidyl transfer (elongation) at a rate of approximately 30 nucleotides/sec. Extracts from hormone-treated cells are capable of forming stable, preinitiation complexes. The rates of stable complex formation are the same in extracts from control and hormone-treated cells. Nevertheless, initiation of transcription does not occur in extracts from hormone-treated cells. Initiation in such extracts may be restored by the addition of a partially purified RNA polymerase I initiation factor, designated TFIC. The data indicate that initiation by RNA polymerase I is a multi-step process. The first step involves the formation of stable, preinitiation complexes, as demonstrated by a number of groups. Initiation, per se, requires an additional protein, TFIC. Glucocorticoids and perhaps other mitogenic agents regulate transcription of rDNA by influencing the amount or activity of TFIC.
AB - Various parameters of transcription of cloned mouse rDNA have been examined using extracts from control P1798 cells and from cells treated 24h with 0.1 μM dexamethasone. Highly purified RNA polymerase I from either source catalyzes nucleotidyl transfer (elongation) at a rate of approximately 30 nucleotides/sec. Extracts from hormone-treated cells are capable of forming stable, preinitiation complexes. The rates of stable complex formation are the same in extracts from control and hormone-treated cells. Nevertheless, initiation of transcription does not occur in extracts from hormone-treated cells. Initiation in such extracts may be restored by the addition of a partially purified RNA polymerase I initiation factor, designated TFIC. The data indicate that initiation by RNA polymerase I is a multi-step process. The first step involves the formation of stable, preinitiation complexes, as demonstrated by a number of groups. Initiation, per se, requires an additional protein, TFIC. Glucocorticoids and perhaps other mitogenic agents regulate transcription of rDNA by influencing the amount or activity of TFIC.
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U2 - 10.1093/nar/13.9.3357
DO - 10.1093/nar/13.9.3357
M3 - Article
C2 - 4000974
AN - SCOPUS:0022422676
SN - 0305-1048
VL - 13
SP - 3357
EP - 3369
JO - Nucleic acids research
JF - Nucleic acids research
IS - 9
ER -