HNRNPH1 is required for rhabdomyosarcoma cell growth and survival

Yanfeng Li, Jesse Bakke, David Finkelstein, Hu Zeng, Jing Wu, Taosheng Chen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Rhabdomyosarcoma (RMS) is an aggressive and difficult to treat cancer characterized by a muscle-like phenotype. Although the average 5-y survival rate is 65% for newly diagnosed RMS, the treatment options for metastatic disease are limited in efficacy, with the 5-y survival rate plummeting to 30%. Heterogenous nuclear ribonucleoprotein H1 (HNRNPH1) is an RNA-binding protein that is highly expressed in many cancers, including RMS. To determine the role HNRNPH1 plays in RMS tumorigenesis, we investigated its expression and effect on growth in three cellular models of RMS: RD, RH30, and RH41 cells. Upon knockdown of HNRNPH1, growth of all cell lines was reduced, most likely through a combination of apoptosis and cell cycle arrest. We then recapitulated this finding by performing in vivo xenograft studies, in which knockdown of HNRNPH1 resulted in a reduction of tumor formation and growth. We used RNA sequencing to identify changes in gene expression after HNRNPH1 knockdown and found altered splicing of some oncogenes. Our data contribute to understanding the role of HNRNPH1 in RMS development.

Original languageEnglish (US)
Article number0024
JournalOncogenesis
Volume7
Issue number1
DOIs
StatePublished - Jan 1 2018
Externally publishedYes

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Ribonucleoproteins
Rhabdomyosarcoma
Cell Survival
Growth
RNA Sequence Analysis
Neoplasms
RNA-Binding Proteins
Cell Cycle Checkpoints
Oncogenes
Heterografts
Carcinogenesis
Apoptosis
Phenotype
Gene Expression
Cell Line
Muscles

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research

Cite this

HNRNPH1 is required for rhabdomyosarcoma cell growth and survival. / Li, Yanfeng; Bakke, Jesse; Finkelstein, David; Zeng, Hu; Wu, Jing; Chen, Taosheng.

In: Oncogenesis, Vol. 7, No. 1, 0024, 01.01.2018.

Research output: Contribution to journalArticle

Li, Yanfeng ; Bakke, Jesse ; Finkelstein, David ; Zeng, Hu ; Wu, Jing ; Chen, Taosheng. / HNRNPH1 is required for rhabdomyosarcoma cell growth and survival. In: Oncogenesis. 2018 ; Vol. 7, No. 1.
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