HMGB Binding to DNA: Single and Double Box Motifs

Micah J. McCauley, Jeff Zimmerman, L James Maher III, Mark C. Williams

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

High mobility group (HMG) proteins are nuclear proteins believed to significantly affect DNA interactions by altering nucleic acid flexibility. Group B (HMGB) proteins contain HMG box domains known to bind to the DNA minor groove without sequence specificity, slightly intercalating base pairs and inducing a strong bend in the DNA helical axis. A dual-beam optical tweezers system is used to extend double-stranded DNA (dsDNA) in the absence as well as presence of a single box derivative of human HMGB2 [HMGB2(box A)] and a double box derivative of rat HMGB1 [HMGB1(box A+box B)]. The single box domain is observed to reduce the persistence length of the double helix, generating sharp DNA bends with an average bending angle of 99 ± 9° and, at very high concentrations, stabilizing dsDNA against denaturation. The double box protein contains two consecutive HMG box domains joined by a flexible tether. This protein also reduces the DNA persistence length, induces an average bending angle of 77 ± 7°, and stabilizes dsDNA at significantly lower concentrations. These results suggest that single and double box proteins increase DNA flexibility and stability, albeit both effects are achieved at much lower protein concentrations for the double box. In addition, at low concentrations, the single box protein can alter DNA flexibility without stabilizing dsDNA, whereas stabilization at higher concentrations is likely achieved through a cooperative binding mode.

Original languageEnglish (US)
Pages (from-to)993-1004
Number of pages12
JournalJournal of Molecular Biology
Volume374
Issue number4
DOIs
StatePublished - Dec 7 2007

Fingerprint

HMGB Proteins
HMG-Box Domains
HMGB2 Protein
HMGB1 Protein
Proteins
DNA
Optical Tweezers
High Mobility Group Proteins
Nucleic Acid Denaturation
Optical Devices
Nuclear Proteins
Base Pairing
Nucleic Acids

Keywords

  • bending angle
  • DNA stretching
  • double box motif
  • single box motif
  • single molecule

ASJC Scopus subject areas

  • Virology

Cite this

HMGB Binding to DNA : Single and Double Box Motifs. / McCauley, Micah J.; Zimmerman, Jeff; Maher III, L James; Williams, Mark C.

In: Journal of Molecular Biology, Vol. 374, No. 4, 07.12.2007, p. 993-1004.

Research output: Contribution to journalArticle

McCauley, Micah J. ; Zimmerman, Jeff ; Maher III, L James ; Williams, Mark C. / HMGB Binding to DNA : Single and Double Box Motifs. In: Journal of Molecular Biology. 2007 ; Vol. 374, No. 4. pp. 993-1004.
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