HLA-DRB1 molecules and antigenic experience shape the repertoire of CD4 T cells

Debby R. Walser-Kuntz; Cornelia M. Weyand; James W. Fulbright; S. Breanndan Moore; Jörg J. Goronzy

doi:10.1016/0198-8859(95)00109-3

HLA-DRB1 molecules and antigenic experience shape the repertoire of CD4 T cells

Debby R. Walser-Kuntz, Cornelia M. Weyand, James W. Fulbright, S. Breanndan Moore, Jörg J. Goronzy

Immunology

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Forces influencing the composition of the mature TCR repertoire have been well studied in the mouse. In particular, the contribution of MHC molecules in negative and positive selection events of T lymphocytes has been established. To understand whether the allelic polymorphism of HLA-DRB1 molecules can shape the human TCR repertoire, we compared the usage of TCR Vβ segments in two cohorts of unrelated individuals who were selected for the expression of HLA-DRB1 alleles. To investigate the potential role of antigenic experience in shaping the TCR repertoire, we compared the usage of Vβ gene elements in CD45RO^- CD4⁺ (naive) T cells versus CD45RO⁺ CD4⁺ (memory) T cells. A correlation between Vβ gene segment usage and HLA-DRB1 alleles could be demonstrated for the repertoire of the naive CD4⁺ T cells, suggesting a shaping force of the HLDRB1 allele on the peripheral TCR repertoire. While the HLA-DRB1 imposed profile in Vβ distribution was maintained in CD45RO⁺ CD4⁺ T cells, it was less pronounced, indicating that antigenic experience modulates the functional TCR repertoire.

Original language	English (US)
Pages (from-to)	203-209
Number of pages	7
Journal	Human Immunology
Volume	44
Issue number	4
DOIs	https://doi.org/10.1016/0198-8859(95)00109-3
State	Published - Dec 1995

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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title = "HLA-DRB1 molecules and antigenic experience shape the repertoire of CD4 T cells",

abstract = "Forces influencing the composition of the mature TCR repertoire have been well studied in the mouse. In particular, the contribution of MHC molecules in negative and positive selection events of T lymphocytes has been established. To understand whether the allelic polymorphism of HLA-DRB1 molecules can shape the human TCR repertoire, we compared the usage of TCR Vβ segments in two cohorts of unrelated individuals who were selected for the expression of HLA-DRB1 alleles. To investigate the potential role of antigenic experience in shaping the TCR repertoire, we compared the usage of Vβ gene elements in CD45RO- CD4+ (naive) T cells versus CD45RO+ CD4+ (memory) T cells. A correlation between Vβ gene segment usage and HLA-DRB1 alleles could be demonstrated for the repertoire of the naive CD4+ T cells, suggesting a shaping force of the HLDRB1 allele on the peripheral TCR repertoire. While the HLA-DRB1 imposed profile in Vβ distribution was maintained in CD45RO+ CD4+ T cells, it was less pronounced, indicating that antigenic experience modulates the functional TCR repertoire.",

author = "Walser-Kuntz, {Debby R.} and Weyand, {Cornelia M.} and Fulbright, {James W.} and Moore, {S. Breanndan} and Goronzy, {J{\"o}rg J.}",

note = "Funding Information: The authors wish to thank Toni L. Higgins for secretarial support. This work was supported in part by the National Institutes of Health (ROl-AR41974, ROI-AR42527, ROl-AR3 1155), a Clinical Science Grant from the National Arthritis Foundation, and by the Mayo Foundation.",

year = "1995",

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doi = "10.1016/0198-8859(95)00109-3",

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AU - Weyand, Cornelia M.

AU - Fulbright, James W.

AU - Moore, S. Breanndan

AU - Goronzy, Jörg J.

N1 - Funding Information: The authors wish to thank Toni L. Higgins for secretarial support. This work was supported in part by the National Institutes of Health (ROl-AR41974, ROI-AR42527, ROl-AR3 1155), a Clinical Science Grant from the National Arthritis Foundation, and by the Mayo Foundation.

PY - 1995/12

Y1 - 1995/12

N2 - Forces influencing the composition of the mature TCR repertoire have been well studied in the mouse. In particular, the contribution of MHC molecules in negative and positive selection events of T lymphocytes has been established. To understand whether the allelic polymorphism of HLA-DRB1 molecules can shape the human TCR repertoire, we compared the usage of TCR Vβ segments in two cohorts of unrelated individuals who were selected for the expression of HLA-DRB1 alleles. To investigate the potential role of antigenic experience in shaping the TCR repertoire, we compared the usage of Vβ gene elements in CD45RO- CD4+ (naive) T cells versus CD45RO+ CD4+ (memory) T cells. A correlation between Vβ gene segment usage and HLA-DRB1 alleles could be demonstrated for the repertoire of the naive CD4+ T cells, suggesting a shaping force of the HLDRB1 allele on the peripheral TCR repertoire. While the HLA-DRB1 imposed profile in Vβ distribution was maintained in CD45RO+ CD4+ T cells, it was less pronounced, indicating that antigenic experience modulates the functional TCR repertoire.

AB - Forces influencing the composition of the mature TCR repertoire have been well studied in the mouse. In particular, the contribution of MHC molecules in negative and positive selection events of T lymphocytes has been established. To understand whether the allelic polymorphism of HLA-DRB1 molecules can shape the human TCR repertoire, we compared the usage of TCR Vβ segments in two cohorts of unrelated individuals who were selected for the expression of HLA-DRB1 alleles. To investigate the potential role of antigenic experience in shaping the TCR repertoire, we compared the usage of Vβ gene elements in CD45RO- CD4+ (naive) T cells versus CD45RO+ CD4+ (memory) T cells. A correlation between Vβ gene segment usage and HLA-DRB1 alleles could be demonstrated for the repertoire of the naive CD4+ T cells, suggesting a shaping force of the HLDRB1 allele on the peripheral TCR repertoire. While the HLA-DRB1 imposed profile in Vβ distribution was maintained in CD45RO+ CD4+ T cells, it was less pronounced, indicating that antigenic experience modulates the functional TCR repertoire.

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HLA-DRB1 molecules and antigenic experience shape the repertoire of CD4 T cells

Abstract

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