HLA-DQ8 transgenic mice lacking endogenous class II molecules respond to house dust allergens: Identification of antigenic epitopes

Teresa Neeno, Christopher J. Krco, Jerry Harders, Jeanine Baisch, Shen Cheng, Chella S. David

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

We have introduced HLA-DQ8 (HLADQB*0302 and HLA-DQA*0301) genes into Aβ0 knockout mice. Transgenic animals were immunized with a whole body extract of Dermatophagoides pteronyssinus (Der p), one of the causative agents of house dust mite allergy. Transgenic mice expressing HLA-DQ8 genes elicited HLA-DQ8-restricted responses driven by CD4+ T cells. Synthetic overlapping peptides representing a major allergen of house dust mite (Der p 2) were synthesized and used as immunogens. HLA-DQ8+ mice responded to three peptides: 8 (residues 61-80), 11 (residues 91-110), and 13 (residues 111129). The mice produced IL-2, IL-4, and IL-6 response to Der p challenge, suggesting a mixed Th1/Th2 response. These mice represent a new model for studies of the immune basis of allergy.

Original languageEnglish (US)
Pages (from-to)3191-3195
Number of pages5
JournalJournal of Immunology
Volume156
Issue number9
StatePublished - May 1 1996

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Neeno, T., Krco, C. J., Harders, J., Baisch, J., Cheng, S., & David, C. S. (1996). HLA-DQ8 transgenic mice lacking endogenous class II molecules respond to house dust allergens: Identification of antigenic epitopes. Journal of Immunology, 156(9), 3191-3195.