TY - JOUR
T1 - HLA-DQ8 transgenic mice lacking endogenous class II molecules respond to house dust allergens
T2 - Identification of antigenic epitopes
AU - Neeno, Teresa
AU - Krco, Christopher J.
AU - Harders, Jerry
AU - Baisch, Jeanine
AU - Cheng, Shen
AU - David, Chella S.
PY - 1996/5/1
Y1 - 1996/5/1
N2 - We have introduced HLA-DQ8 (HLADQB*0302 and HLA-DQA*0301) genes into Aβ0 knockout mice. Transgenic animals were immunized with a whole body extract of Dermatophagoides pteronyssinus (Der p), one of the causative agents of house dust mite allergy. Transgenic mice expressing HLA-DQ8 genes elicited HLA-DQ8-restricted responses driven by CD4+ T cells. Synthetic overlapping peptides representing a major allergen of house dust mite (Der p 2) were synthesized and used as immunogens. HLA-DQ8+ mice responded to three peptides: 8 (residues 61-80), 11 (residues 91-110), and 13 (residues 111129). The mice produced IL-2, IL-4, and IL-6 response to Der p challenge, suggesting a mixed Th1/Th2 response. These mice represent a new model for studies of the immune basis of allergy.
AB - We have introduced HLA-DQ8 (HLADQB*0302 and HLA-DQA*0301) genes into Aβ0 knockout mice. Transgenic animals were immunized with a whole body extract of Dermatophagoides pteronyssinus (Der p), one of the causative agents of house dust mite allergy. Transgenic mice expressing HLA-DQ8 genes elicited HLA-DQ8-restricted responses driven by CD4+ T cells. Synthetic overlapping peptides representing a major allergen of house dust mite (Der p 2) were synthesized and used as immunogens. HLA-DQ8+ mice responded to three peptides: 8 (residues 61-80), 11 (residues 91-110), and 13 (residues 111129). The mice produced IL-2, IL-4, and IL-6 response to Der p challenge, suggesting a mixed Th1/Th2 response. These mice represent a new model for studies of the immune basis of allergy.
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M3 - Article
C2 - 8617940
AN - SCOPUS:0029945679
SN - 0022-1767
VL - 156
SP - 3191
EP - 3195
JO - Journal of Immunology
JF - Journal of Immunology
IS - 9
ER -