HLA-A0201 positive pancreatic cell lines: New findings and discrepancies

Kuichun Zhu, Gregory Lizee, Pedro Cano, Marcelo Fernando-Vina, Baoan Ji, James L. Abbruzzese, Patrick Hwu, Laszlo Radvanyi, David Z. Chang

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Abstract

Pancreatic cancer is being pursued as an immunotherapy target using antigen-specific vaccine approaches activating CD8+ CTL and CD4 + T-helper cells. CD8+ CTL exert their anti-tumor effects in an HLA-restricted manner and only tumor cells carrying a matched HLA class I sub-type are targets for antigen-specific CTL. In the process of characterizing CD8+ T cell responses against pancreatic cancer, we screened a number of human pancreatic tumor cell lines for HLA-A0201 positive (HLA-A2 +) cell lines to be used in the evaluation of CTL function. This analysis revealed some new findings and discrepancies in the literature on the HLA sub-type of some commonly used pancreatic cell lines. We found that Capan-1 cells, originally reported to be HLA-A0201+, actually only express HLA-A010101 and HLA-A300101 and were targets for HLA-A0201-restricted CTL only after transduction with an HLA-A0201-expressing lentivirus. Panc-1 cells were found to be HLA-A0201 positive, in agreement with published reports, while CF-Pac-1 cells were found to express both HLA-A020101 and HLA-A030101. We also found a normal human pancreatic ductal epithelial cell line, HPDE, to be HLA-A0201 positive. Our findings were verified with two different sequence-based typing methods, antibody staining followed by flow cytometry analysis, and functional analysis using an HLA-A0201-restricted peptide-specific T cell response.

Original languageEnglish (US)
Pages (from-to)719-724
Number of pages6
JournalCancer Immunology, Immunotherapy
Volume56
Issue number5
DOIs
StatePublished - May 1 2007

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Keywords

  • CD8 T cells
  • CF Pac-1
  • Capan-1
  • HLA-A0201
  • HPDE
  • Pancreatic cancer

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Oncology
  • Cancer Research

Cite this

Zhu, K., Lizee, G., Cano, P., Fernando-Vina, M., Ji, B., Abbruzzese, J. L., Hwu, P., Radvanyi, L., & Chang, D. Z. (2007). HLA-A0201 positive pancreatic cell lines: New findings and discrepancies. Cancer Immunology, Immunotherapy, 56(5), 719-724. https://doi.org/10.1007/s00262-006-0217-8