HLA-A0201 positive pancreatic cell lines: New findings and discrepancies

Kuichun Zhu, Gregory Lizee, Pedro Cano, Marcelo Fernando-Vina, Baoan D Ji, James L. Abbruzzese, Patrick Hwu, Laszlo Radvanyi, David Z. Chang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Pancreatic cancer is being pursued as an immunotherapy target using antigen-specific vaccine approaches activating CD8+ CTL and CD4 + T-helper cells. CD8+ CTL exert their anti-tumor effects in an HLA-restricted manner and only tumor cells carrying a matched HLA class I sub-type are targets for antigen-specific CTL. In the process of characterizing CD8+ T cell responses against pancreatic cancer, we screened a number of human pancreatic tumor cell lines for HLA-A0201 positive (HLA-A2 +) cell lines to be used in the evaluation of CTL function. This analysis revealed some new findings and discrepancies in the literature on the HLA sub-type of some commonly used pancreatic cell lines. We found that Capan-1 cells, originally reported to be HLA-A0201+, actually only express HLA-A010101 and HLA-A300101 and were targets for HLA-A0201-restricted CTL only after transduction with an HLA-A0201-expressing lentivirus. Panc-1 cells were found to be HLA-A0201 positive, in agreement with published reports, while CF-Pac-1 cells were found to express both HLA-A020101 and HLA-A030101. We also found a normal human pancreatic ductal epithelial cell line, HPDE, to be HLA-A0201 positive. Our findings were verified with two different sequence-based typing methods, antibody staining followed by flow cytometry analysis, and functional analysis using an HLA-A0201-restricted peptide-specific T cell response.

Original languageEnglish (US)
Pages (from-to)719-724
Number of pages6
JournalCancer Immunology, Immunotherapy
Volume56
Issue number5
DOIs
StatePublished - May 2007
Externally publishedYes

Fingerprint

Cell Line
Pancreatic Neoplasms
Peptide T
T-Lymphocytes
HLA-A2 Antigen
Antigens
Lentivirus
Helper-Inducer T-Lymphocytes
HLA-A*02:01 antigen
Tumor Cell Line
Immunotherapy
Neoplasms
Flow Cytometry
Vaccines
Epithelial Cells
Staining and Labeling
Antibodies

Keywords

  • Capan-1
  • CD8 T cells
  • CF Pac-1
  • HLA-A0201
  • HPDE
  • Pancreatic cancer

ASJC Scopus subject areas

  • Cancer Research
  • Immunology
  • Oncology

Cite this

Zhu, K., Lizee, G., Cano, P., Fernando-Vina, M., Ji, B. D., Abbruzzese, J. L., ... Chang, D. Z. (2007). HLA-A0201 positive pancreatic cell lines: New findings and discrepancies. Cancer Immunology, Immunotherapy, 56(5), 719-724. https://doi.org/10.1007/s00262-006-0217-8

HLA-A0201 positive pancreatic cell lines : New findings and discrepancies. / Zhu, Kuichun; Lizee, Gregory; Cano, Pedro; Fernando-Vina, Marcelo; Ji, Baoan D; Abbruzzese, James L.; Hwu, Patrick; Radvanyi, Laszlo; Chang, David Z.

In: Cancer Immunology, Immunotherapy, Vol. 56, No. 5, 05.2007, p. 719-724.

Research output: Contribution to journalArticle

Zhu, K, Lizee, G, Cano, P, Fernando-Vina, M, Ji, BD, Abbruzzese, JL, Hwu, P, Radvanyi, L & Chang, DZ 2007, 'HLA-A0201 positive pancreatic cell lines: New findings and discrepancies', Cancer Immunology, Immunotherapy, vol. 56, no. 5, pp. 719-724. https://doi.org/10.1007/s00262-006-0217-8
Zhu, Kuichun ; Lizee, Gregory ; Cano, Pedro ; Fernando-Vina, Marcelo ; Ji, Baoan D ; Abbruzzese, James L. ; Hwu, Patrick ; Radvanyi, Laszlo ; Chang, David Z. / HLA-A0201 positive pancreatic cell lines : New findings and discrepancies. In: Cancer Immunology, Immunotherapy. 2007 ; Vol. 56, No. 5. pp. 719-724.
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