Histopathology and immunohistochemistry in distinguishing Burkitt lymphoma from diffuse large B-cell lymphoma with very high proliferation index and with or without a starry-sky pattern: A comparative study with EBER and FISH

Shih Sung Chuang, Hongtao Ye, Ming Qing Du, Chin Li Lu, Ahmet Dogan, Pin Pen Hsieh, Wan Ting Huang, Yun Chih Jung

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Burkitt lymphoma (BL) is characterized by c-myc translocation and CD10+/bcl-2-/bcl-6+ with a very high Ki-67 proliferation index (PI). Occasional diffuse large B-cell lymphomas may exhibit a very high PI with or without a starry-sky pattern (DLBCL-HPSS). We compared 28 consecutive BL and 16 DLBCL-HPSS cases in immunocompetent Taiwanese diagnosed by histopathologic examination and immunophenotyping and compared the results with results for Epstein-Barr virus-encoded messenger RNA (EBER) and fluorescence in situ hybridization (FISH). There were statistically significant differences in the expression of CD10 (28/28 vs 1/16), bcl-2 (3/28 vs 11/16), MUM1 (5/28 vs 15/16), a PI of 95.0% or more (27/28 vs 2/16), and combined CD10+/bcl-2-/bcl-6+ (24/28 vs 1/16) between BLs and DLBCL-HPSSs. Of the BLs, 7 (25%) of 28 and 26 (96%) of 27 were positive for EBER and c-myc rearrangement as compared with 0 of 16 and 1 (7%) of 15 DLBCL-HPSSs, respectively. We can confidently distinguish BL from DLBCL-HPSS by using histopathologic and immunohistochemical (CD10, bcl-2, bcl-6, Ki-67) methods without the aid of EBER and FISH in the great majority of cases.

Original languageEnglish (US)
Pages (from-to)558-564
Number of pages7
JournalAmerican Journal of Clinical Pathology
Volume128
Issue number4
DOIs
StatePublished - Oct 2007

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Burkitt Lymphoma
Lymphoma, Large B-Cell, Diffuse
Fluorescence In Situ Hybridization
Immunohistochemistry
Immunophenotyping
Human Herpesvirus 4
Messenger RNA

Keywords

  • Burkitt lymphoma
  • Diffuse large B-cell lymphoma
  • Epstein-Barr virus
  • Fluorescence in situ hybridization
  • Taiwan

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Histopathology and immunohistochemistry in distinguishing Burkitt lymphoma from diffuse large B-cell lymphoma with very high proliferation index and with or without a starry-sky pattern : A comparative study with EBER and FISH. / Chuang, Shih Sung; Ye, Hongtao; Du, Ming Qing; Lu, Chin Li; Dogan, Ahmet; Hsieh, Pin Pen; Huang, Wan Ting; Jung, Yun Chih.

In: American Journal of Clinical Pathology, Vol. 128, No. 4, 10.2007, p. 558-564.

Research output: Contribution to journalArticle

Chuang, Shih Sung ; Ye, Hongtao ; Du, Ming Qing ; Lu, Chin Li ; Dogan, Ahmet ; Hsieh, Pin Pen ; Huang, Wan Ting ; Jung, Yun Chih. / Histopathology and immunohistochemistry in distinguishing Burkitt lymphoma from diffuse large B-cell lymphoma with very high proliferation index and with or without a starry-sky pattern : A comparative study with EBER and FISH. In: American Journal of Clinical Pathology. 2007 ; Vol. 128, No. 4. pp. 558-564.
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abstract = "Burkitt lymphoma (BL) is characterized by c-myc translocation and CD10+/bcl-2-/bcl-6+ with a very high Ki-67 proliferation index (PI). Occasional diffuse large B-cell lymphomas may exhibit a very high PI with or without a starry-sky pattern (DLBCL-HPSS). We compared 28 consecutive BL and 16 DLBCL-HPSS cases in immunocompetent Taiwanese diagnosed by histopathologic examination and immunophenotyping and compared the results with results for Epstein-Barr virus-encoded messenger RNA (EBER) and fluorescence in situ hybridization (FISH). There were statistically significant differences in the expression of CD10 (28/28 vs 1/16), bcl-2 (3/28 vs 11/16), MUM1 (5/28 vs 15/16), a PI of 95.0{\%} or more (27/28 vs 2/16), and combined CD10+/bcl-2-/bcl-6+ (24/28 vs 1/16) between BLs and DLBCL-HPSSs. Of the BLs, 7 (25{\%}) of 28 and 26 (96{\%}) of 27 were positive for EBER and c-myc rearrangement as compared with 0 of 16 and 1 (7{\%}) of 15 DLBCL-HPSSs, respectively. We can confidently distinguish BL from DLBCL-HPSS by using histopathologic and immunohistochemical (CD10, bcl-2, bcl-6, Ki-67) methods without the aid of EBER and FISH in the great majority of cases.",
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T1 - Histopathology and immunohistochemistry in distinguishing Burkitt lymphoma from diffuse large B-cell lymphoma with very high proliferation index and with or without a starry-sky pattern

T2 - A comparative study with EBER and FISH

AU - Chuang, Shih Sung

AU - Ye, Hongtao

AU - Du, Ming Qing

AU - Lu, Chin Li

AU - Dogan, Ahmet

AU - Hsieh, Pin Pen

AU - Huang, Wan Ting

AU - Jung, Yun Chih

PY - 2007/10

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N2 - Burkitt lymphoma (BL) is characterized by c-myc translocation and CD10+/bcl-2-/bcl-6+ with a very high Ki-67 proliferation index (PI). Occasional diffuse large B-cell lymphomas may exhibit a very high PI with or without a starry-sky pattern (DLBCL-HPSS). We compared 28 consecutive BL and 16 DLBCL-HPSS cases in immunocompetent Taiwanese diagnosed by histopathologic examination and immunophenotyping and compared the results with results for Epstein-Barr virus-encoded messenger RNA (EBER) and fluorescence in situ hybridization (FISH). There were statistically significant differences in the expression of CD10 (28/28 vs 1/16), bcl-2 (3/28 vs 11/16), MUM1 (5/28 vs 15/16), a PI of 95.0% or more (27/28 vs 2/16), and combined CD10+/bcl-2-/bcl-6+ (24/28 vs 1/16) between BLs and DLBCL-HPSSs. Of the BLs, 7 (25%) of 28 and 26 (96%) of 27 were positive for EBER and c-myc rearrangement as compared with 0 of 16 and 1 (7%) of 15 DLBCL-HPSSs, respectively. We can confidently distinguish BL from DLBCL-HPSS by using histopathologic and immunohistochemical (CD10, bcl-2, bcl-6, Ki-67) methods without the aid of EBER and FISH in the great majority of cases.

AB - Burkitt lymphoma (BL) is characterized by c-myc translocation and CD10+/bcl-2-/bcl-6+ with a very high Ki-67 proliferation index (PI). Occasional diffuse large B-cell lymphomas may exhibit a very high PI with or without a starry-sky pattern (DLBCL-HPSS). We compared 28 consecutive BL and 16 DLBCL-HPSS cases in immunocompetent Taiwanese diagnosed by histopathologic examination and immunophenotyping and compared the results with results for Epstein-Barr virus-encoded messenger RNA (EBER) and fluorescence in situ hybridization (FISH). There were statistically significant differences in the expression of CD10 (28/28 vs 1/16), bcl-2 (3/28 vs 11/16), MUM1 (5/28 vs 15/16), a PI of 95.0% or more (27/28 vs 2/16), and combined CD10+/bcl-2-/bcl-6+ (24/28 vs 1/16) between BLs and DLBCL-HPSSs. Of the BLs, 7 (25%) of 28 and 26 (96%) of 27 were positive for EBER and c-myc rearrangement as compared with 0 of 16 and 1 (7%) of 15 DLBCL-HPSSs, respectively. We can confidently distinguish BL from DLBCL-HPSS by using histopathologic and immunohistochemical (CD10, bcl-2, bcl-6, Ki-67) methods without the aid of EBER and FISH in the great majority of cases.

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KW - Fluorescence in situ hybridization

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