Histopathologic parameters and DNA analysis in colorectal adenocarcinomas.

J. D. Crissman, R. J. Zarbo, C. K. Ma, Daniel W Visscher

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Human colon adenocarcinomas have histological parameters that are clearly associated with prognosis. These include tumor grade, pattern of invasion, presence of lymphocytes, and vascular involvement by tumor. The latter remains controversial with respect to the relative importance of intramural and extramural vascular involvement. Some studies show a poor prognosis for intramural invasion of capillary size vascular channels by tumor. On the other hand, when veins are involved by tumor, the presence of tumor in large extramural veins appears to have a much more ominous effect than intramural tumor involvement of small veins. The results of DNA analysis of colorectal adenocarcinomas varied greatly depending on study methodology but several important points can be summarized: (1) higher stage tumors have a greater proportion of aneuploid tumors; (2) aneuploid tumors tend to have a higher growth rate (SPF) and poorer survival than diploid tumors; and (3) aneuploid tumors are associated with histological parameters indicative of a poor prognosis such as vascular invasion, but ploidy is not related to tumor grade. One of the major problems in drawing firm conclusions about the relationship of flow cytometric DNA measurements to prognosis is great variability among the reported studies. The types of variation appear to fall into two major categories: patient selection and technical problems. The former are especially relevant in retrospective studies in which there is poor patient definition (site, grade, stage, and other standard tumor definitions) and a bias for selecting early stage tumors which have improved survivals. The latter includes a spectrum of technical problems inherent in this widely but not necessarily uniformly applied laboratory procedure. This is particularly true for DNA analysis of nuclei removed from paraffin tissue blocks. For the most part, quality control measurements including cell yields, the efficiency of extraction or disaggregation of aneuploid cells, exclusion of non-neoplastic cells, and other features characteristic of the scientific method are seldom included in studies of DNA analysis of solid tumors. Hopefully, a consensus regarding optimum technical and analytic methods will evolve, followed by the careful definition of human colon cancer cohorts. The importance of further studies is suggested by the results of this review which indicated that the presence of an aneuploid cell population is associated with a less favorable prognosis for all colorectal tumors.

Original languageEnglish (US)
Pages (from-to)103-147
Number of pages45
JournalPathology Annual
Volume24 Pt 2
StatePublished - 1989
Externally publishedYes

Fingerprint

Adenocarcinoma
DNA
Neoplasms
Aneuploidy
Blood Vessels
Veins
Survival
Ploidies
Diploidy
Quality Control
Paraffin
Colonic Neoplasms
Patient Selection
Colorectal Neoplasms
Colon
Retrospective Studies
Lymphocytes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Histopathologic parameters and DNA analysis in colorectal adenocarcinomas. / Crissman, J. D.; Zarbo, R. J.; Ma, C. K.; Visscher, Daniel W.

In: Pathology Annual, Vol. 24 Pt 2, 1989, p. 103-147.

Research output: Contribution to journalArticle

Crissman, JD, Zarbo, RJ, Ma, CK & Visscher, DW 1989, 'Histopathologic parameters and DNA analysis in colorectal adenocarcinomas.', Pathology Annual, vol. 24 Pt 2, pp. 103-147.
Crissman, J. D. ; Zarbo, R. J. ; Ma, C. K. ; Visscher, Daniel W. / Histopathologic parameters and DNA analysis in colorectal adenocarcinomas. In: Pathology Annual. 1989 ; Vol. 24 Pt 2. pp. 103-147.
@article{6c915d865f7d4f6d9170737fd1ca369c,
title = "Histopathologic parameters and DNA analysis in colorectal adenocarcinomas.",
abstract = "Human colon adenocarcinomas have histological parameters that are clearly associated with prognosis. These include tumor grade, pattern of invasion, presence of lymphocytes, and vascular involvement by tumor. The latter remains controversial with respect to the relative importance of intramural and extramural vascular involvement. Some studies show a poor prognosis for intramural invasion of capillary size vascular channels by tumor. On the other hand, when veins are involved by tumor, the presence of tumor in large extramural veins appears to have a much more ominous effect than intramural tumor involvement of small veins. The results of DNA analysis of colorectal adenocarcinomas varied greatly depending on study methodology but several important points can be summarized: (1) higher stage tumors have a greater proportion of aneuploid tumors; (2) aneuploid tumors tend to have a higher growth rate (SPF) and poorer survival than diploid tumors; and (3) aneuploid tumors are associated with histological parameters indicative of a poor prognosis such as vascular invasion, but ploidy is not related to tumor grade. One of the major problems in drawing firm conclusions about the relationship of flow cytometric DNA measurements to prognosis is great variability among the reported studies. The types of variation appear to fall into two major categories: patient selection and technical problems. The former are especially relevant in retrospective studies in which there is poor patient definition (site, grade, stage, and other standard tumor definitions) and a bias for selecting early stage tumors which have improved survivals. The latter includes a spectrum of technical problems inherent in this widely but not necessarily uniformly applied laboratory procedure. This is particularly true for DNA analysis of nuclei removed from paraffin tissue blocks. For the most part, quality control measurements including cell yields, the efficiency of extraction or disaggregation of aneuploid cells, exclusion of non-neoplastic cells, and other features characteristic of the scientific method are seldom included in studies of DNA analysis of solid tumors. Hopefully, a consensus regarding optimum technical and analytic methods will evolve, followed by the careful definition of human colon cancer cohorts. The importance of further studies is suggested by the results of this review which indicated that the presence of an aneuploid cell population is associated with a less favorable prognosis for all colorectal tumors.",
author = "Crissman, {J. D.} and Zarbo, {R. J.} and Ma, {C. K.} and Visscher, {Daniel W}",
year = "1989",
language = "English (US)",
volume = "24 Pt 2",
pages = "103--147",
journal = "Pathology Annual",
issn = "0079-0184",
publisher = "Appleton & Lange",

}

TY - JOUR

T1 - Histopathologic parameters and DNA analysis in colorectal adenocarcinomas.

AU - Crissman, J. D.

AU - Zarbo, R. J.

AU - Ma, C. K.

AU - Visscher, Daniel W

PY - 1989

Y1 - 1989

N2 - Human colon adenocarcinomas have histological parameters that are clearly associated with prognosis. These include tumor grade, pattern of invasion, presence of lymphocytes, and vascular involvement by tumor. The latter remains controversial with respect to the relative importance of intramural and extramural vascular involvement. Some studies show a poor prognosis for intramural invasion of capillary size vascular channels by tumor. On the other hand, when veins are involved by tumor, the presence of tumor in large extramural veins appears to have a much more ominous effect than intramural tumor involvement of small veins. The results of DNA analysis of colorectal adenocarcinomas varied greatly depending on study methodology but several important points can be summarized: (1) higher stage tumors have a greater proportion of aneuploid tumors; (2) aneuploid tumors tend to have a higher growth rate (SPF) and poorer survival than diploid tumors; and (3) aneuploid tumors are associated with histological parameters indicative of a poor prognosis such as vascular invasion, but ploidy is not related to tumor grade. One of the major problems in drawing firm conclusions about the relationship of flow cytometric DNA measurements to prognosis is great variability among the reported studies. The types of variation appear to fall into two major categories: patient selection and technical problems. The former are especially relevant in retrospective studies in which there is poor patient definition (site, grade, stage, and other standard tumor definitions) and a bias for selecting early stage tumors which have improved survivals. The latter includes a spectrum of technical problems inherent in this widely but not necessarily uniformly applied laboratory procedure. This is particularly true for DNA analysis of nuclei removed from paraffin tissue blocks. For the most part, quality control measurements including cell yields, the efficiency of extraction or disaggregation of aneuploid cells, exclusion of non-neoplastic cells, and other features characteristic of the scientific method are seldom included in studies of DNA analysis of solid tumors. Hopefully, a consensus regarding optimum technical and analytic methods will evolve, followed by the careful definition of human colon cancer cohorts. The importance of further studies is suggested by the results of this review which indicated that the presence of an aneuploid cell population is associated with a less favorable prognosis for all colorectal tumors.

AB - Human colon adenocarcinomas have histological parameters that are clearly associated with prognosis. These include tumor grade, pattern of invasion, presence of lymphocytes, and vascular involvement by tumor. The latter remains controversial with respect to the relative importance of intramural and extramural vascular involvement. Some studies show a poor prognosis for intramural invasion of capillary size vascular channels by tumor. On the other hand, when veins are involved by tumor, the presence of tumor in large extramural veins appears to have a much more ominous effect than intramural tumor involvement of small veins. The results of DNA analysis of colorectal adenocarcinomas varied greatly depending on study methodology but several important points can be summarized: (1) higher stage tumors have a greater proportion of aneuploid tumors; (2) aneuploid tumors tend to have a higher growth rate (SPF) and poorer survival than diploid tumors; and (3) aneuploid tumors are associated with histological parameters indicative of a poor prognosis such as vascular invasion, but ploidy is not related to tumor grade. One of the major problems in drawing firm conclusions about the relationship of flow cytometric DNA measurements to prognosis is great variability among the reported studies. The types of variation appear to fall into two major categories: patient selection and technical problems. The former are especially relevant in retrospective studies in which there is poor patient definition (site, grade, stage, and other standard tumor definitions) and a bias for selecting early stage tumors which have improved survivals. The latter includes a spectrum of technical problems inherent in this widely but not necessarily uniformly applied laboratory procedure. This is particularly true for DNA analysis of nuclei removed from paraffin tissue blocks. For the most part, quality control measurements including cell yields, the efficiency of extraction or disaggregation of aneuploid cells, exclusion of non-neoplastic cells, and other features characteristic of the scientific method are seldom included in studies of DNA analysis of solid tumors. Hopefully, a consensus regarding optimum technical and analytic methods will evolve, followed by the careful definition of human colon cancer cohorts. The importance of further studies is suggested by the results of this review which indicated that the presence of an aneuploid cell population is associated with a less favorable prognosis for all colorectal tumors.

UR - http://www.scopus.com/inward/record.url?scp=0024407980&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024407980&partnerID=8YFLogxK

M3 - Article

VL - 24 Pt 2

SP - 103

EP - 147

JO - Pathology Annual

JF - Pathology Annual

SN - 0079-0184

ER -