Histopathologic findings in the areas of orange pigment overlying choroidal melanomas

Maria D. Garcia, Diva R. Salomao, Alan D Marmorstein, Jose S Pulido

Research output: Contribution to journalArticle

Abstract

Purpose: Orange pigment is an important sign of malignancy in melanocytic tumors. There is a question as to whether the pigment accumulation is inside of macrophages or retinal pigment epithelial (RPE) cells. We investigated which cells are involved with this color alteration. Methods: We examined enucleated specimens from two patients with choroidal melanoma and dense orange pigment on fundus examination. Color fundus and fundus autofluorescence (FAF) photographs were reviewed followed by examination with fluorescent microscopy, electron microscopy, and immunohistochemistry of enucleated eyes for the specific areas corresponding to the orange pigment. Results: Orange pigment was observed on color fundus photography and correlated with areas of hyperautofluorescence on FAF. Fluorescent microscopy of sections of the enucleated eyes showed autofluorescence in the RPE, which were most pronounced where there was a localized retinal detachment and reactive hyperplasia of the RPE. Immunohistochemical studies were done with keratin (OSCAR and AE1/AE3) and S-100 stained RPE cells, which still were attached to Bruch's membrane. Histiocytes present in the detached retina stained with anti-CD163 antibody and did not show autofluorescence. Electron microscopy studies of the same areas showed the presence of lipofuscin and melanolipofuscin within the clustered RPE cells. Conclusions: Orange pigment in choroidal melanocytic lesions originates from the RPE cells, rather than macrophages, and is most abundant where there is proliferation of the RPE. Translational Relevance: The orange pigment tumoral biomarker arises and is in the retinal pigment epithelium.

Original languageEnglish (US)
Article number4
JournalTranslational Vision Science and Technology
Volume5
Issue number3
DOIs
StatePublished - May 1 2016

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Retinal Pigments
Pigments
Melanoma
Epithelial Cells
Color
Microscopy
Electron Microscopy
Macrophages
Bruch Membrane
Lipofuscin
Histiocytes
Retinal Pigment Epithelium
Photography
Retinal Detachment
Keratins
Hyperplasia
Retina
Electron microscopy
Anti-Idiotypic Antibodies
Neoplasms

Keywords

  • Choroidal melanoma
  • Lipofuscin
  • Melanin
  • Melanolipofuscin
  • Orange pigment
  • Retinal pigment epithelium
  • Uveal melanoma

ASJC Scopus subject areas

  • Biomedical Engineering
  • Ophthalmology

Cite this

Histopathologic findings in the areas of orange pigment overlying choroidal melanomas. / Garcia, Maria D.; Salomao, Diva R.; Marmorstein, Alan D; Pulido, Jose S.

In: Translational Vision Science and Technology, Vol. 5, No. 3, 4, 01.05.2016.

Research output: Contribution to journalArticle

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