Histone deacetylase inhibition promotes osteoblast maturation by altering the histone H4 epigenome and reduces akt phosphorylation

Amel Dudakovic, Jared M. Evans, Ying Li, Sumit Middha, Meghan E. McGee-Lawrence, Andre J van Wijnen, Jennifer J Westendorf

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Background:Histone deacetylase (HDAC) inhibition promotes bone formationin vitrovia undefined epigenetic events. Results:Microarray and ChIP-Seq analyses revealed genomic areas where H4 acetylation is altered by HDAC inhibitors and identified differentially regulated genes. Conclusion:Suberoylanilide hydroxamic acid increases H4 acetylation and suppresses phosphorylation of insulin/Akt signaling mediators in osteoblasts. Significance:Epigenetic profiling is a powerful means to gain mechanistic insights into bone anabolic processes.

Original languageEnglish (US)
Pages (from-to)28783-28791
Number of pages9
JournalJournal of Biological Chemistry
Volume288
Issue number40
DOIs
StatePublished - Oct 4 2013

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Acetylation
Phosphorylation
Histone Deacetylases
Osteoblasts
Epigenomics
Histones
Bone
Bone and Bones
Histone Deacetylase Inhibitors
Microarrays
Genes
Insulin
vorinostat

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Histone deacetylase inhibition promotes osteoblast maturation by altering the histone H4 epigenome and reduces akt phosphorylation. / Dudakovic, Amel; Evans, Jared M.; Li, Ying; Middha, Sumit; McGee-Lawrence, Meghan E.; van Wijnen, Andre J; Westendorf, Jennifer J.

In: Journal of Biological Chemistry, Vol. 288, No. 40, 04.10.2013, p. 28783-28791.

Research output: Contribution to journalArticle

Dudakovic, Amel ; Evans, Jared M. ; Li, Ying ; Middha, Sumit ; McGee-Lawrence, Meghan E. ; van Wijnen, Andre J ; Westendorf, Jennifer J. / Histone deacetylase inhibition promotes osteoblast maturation by altering the histone H4 epigenome and reduces akt phosphorylation. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 40. pp. 28783-28791.
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