Histone deacetylase inhibition promotes osteoblast maturation by altering the histone H4 epigenome and reduces akt phosphorylation

Amel Dudakovic; Jared M. Evans; Ying Li; Sumit Middha; Meghan E. McGee-Lawrence; Andre J. Van Wijnen; Jennifer J. Westendorf

doi:10.1074/jbc.M113.489732

Histone deacetylase inhibition promotes osteoblast maturation by altering the histone H4 epigenome and reduces akt phosphorylation

Amel Dudakovic, Jared M. Evans, Ying Li, Sumit Middha, Meghan E. McGee-Lawrence, Andre J. Van Wijnen, Jennifer J. Westendorf

Orthopedic Surgery

Research output: Contribution to journal › Article

47 Scopus citations

Abstract

Background:Histone deacetylase (HDAC) inhibition promotes bone formationin vitrovia undefined epigenetic events. Results:Microarray and ChIP-Seq analyses revealed genomic areas where H4 acetylation is altered by HDAC inhibitors and identified differentially regulated genes. Conclusion:Suberoylanilide hydroxamic acid increases H4 acetylation and suppresses phosphorylation of insulin/Akt signaling mediators in osteoblasts. Significance:Epigenetic profiling is a powerful means to gain mechanistic insights into bone anabolic processes.

Original language	English (US)
Pages (from-to)	28783-28791
Number of pages	9
Journal	Journal of Biological Chemistry
Volume	288
Issue number	40
DOIs	https://doi.org/10.1074/jbc.M113.489732
State	Published - Oct 4 2013

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ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

Cite this

Dudakovic, A., Evans, J. M., Li, Y., Middha, S., McGee-Lawrence, M. E., Van Wijnen, A. J., & Westendorf, J. J. (2013). Histone deacetylase inhibition promotes osteoblast maturation by altering the histone H4 epigenome and reduces akt phosphorylation. Journal of Biological Chemistry, 288(40), 28783-28791. https://doi.org/10.1074/jbc.M113.489732

Histone deacetylase inhibition promotes osteoblast maturation by altering the histone H4 epigenome and reduces akt phosphorylation. / Dudakovic, Amel; Evans, Jared M.; Li, Ying; Middha, Sumit; McGee-Lawrence, Meghan E.; Van Wijnen, Andre J.; Westendorf, Jennifer J.

In: Journal of Biological Chemistry, Vol. 288, No. 40, 04.10.2013, p. 28783-28791.

Research output: Contribution to journal › Article

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10.1074/jbc.M113.489732