Histological studies of gene-ablated mice support important functional roles for natural killer cells in the uterus during pregnancy

B. Anne Croy, Ali A. Ashkar, Robert A. Foster, James P. DiSanto, Jeanne Magram, Daniel Carson, Sandra J Gendler, Michael J. Grusby, Norbert Wagner, Werner Muller, Marie Josée Guimond

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Maternal lymphocytes having a large and granulated morphology accumulate at healthy implantation sites in normal mice. Insight into the functions of these cells has come from a previous study of two independent lines of mice deficient in natural killer (NK) cells. In pregnant Tgε26 mice, vascular pathology was found that led to the major complications of either fetal death or intrauterine growth retardation. In pregnant p56(lck) null x IL-2Rβ null mice, extensive distension of the decidua was observed that separated the placenta from the myometrium and appeared to be interstitial edema. To strengthen assignment of uterine large granulated lymphocytes to the NK cell lineage and to understand which aspects of NK cell biology may be important for a uterine-based, pregnancy-associated subset, mid-gestation implantation sites from a new series of mice having gene deletions which alter NK cells (IL-2Rγ null, Stat4 null, IL-12 p40 null, β7 integrin null and Muc-1 null) have been examined histologically. The findings support the assignment of pregnancy-associated large granulated cells of mice to the NK cell lineage and suggest that the primary functions of these tissue-based NK cells are to support normal development of the decidua and/or its vasculature using pathways that involve IL-12 mediated signal transduction.

Original languageEnglish (US)
Pages (from-to)111-133
Number of pages23
JournalJournal of Reproductive Immunology
Volume35
Issue number2
DOIs
StatePublished - Nov 15 1997

Fingerprint

Natural Killer Cells
Uterus
Pregnancy
Genes
Decidua
Fetal Growth Retardation
Cell Lineage
Interleukin-12
Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
Lymphocytes
Fetal Death
Myometrium
Gene Deletion
Integrins
Placenta
Blood Vessels
Cell Biology
Signal Transduction
Edema
Mothers

Keywords

  • Decidua
  • Integrins
  • Interleukins
  • Mucins
  • Murine pregnancy
  • Placental blood vessels
  • Uterine lymphocytes

ASJC Scopus subject areas

  • Immunology
  • Reproductive Medicine

Cite this

Histological studies of gene-ablated mice support important functional roles for natural killer cells in the uterus during pregnancy. / Croy, B. Anne; Ashkar, Ali A.; Foster, Robert A.; DiSanto, James P.; Magram, Jeanne; Carson, Daniel; Gendler, Sandra J; Grusby, Michael J.; Wagner, Norbert; Muller, Werner; Guimond, Marie Josée.

In: Journal of Reproductive Immunology, Vol. 35, No. 2, 15.11.1997, p. 111-133.

Research output: Contribution to journalArticle

Croy, BA, Ashkar, AA, Foster, RA, DiSanto, JP, Magram, J, Carson, D, Gendler, SJ, Grusby, MJ, Wagner, N, Muller, W & Guimond, MJ 1997, 'Histological studies of gene-ablated mice support important functional roles for natural killer cells in the uterus during pregnancy', Journal of Reproductive Immunology, vol. 35, no. 2, pp. 111-133. https://doi.org/10.1016/S0165-0378(97)00054-5
Croy, B. Anne ; Ashkar, Ali A. ; Foster, Robert A. ; DiSanto, James P. ; Magram, Jeanne ; Carson, Daniel ; Gendler, Sandra J ; Grusby, Michael J. ; Wagner, Norbert ; Muller, Werner ; Guimond, Marie Josée. / Histological studies of gene-ablated mice support important functional roles for natural killer cells in the uterus during pregnancy. In: Journal of Reproductive Immunology. 1997 ; Vol. 35, No. 2. pp. 111-133.
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