Histologic characteristics of vaginal cuff tissue from patients with vaginal cuff dehiscence

Jennifer Klauschie, Yan Wen, Bertha Chen, Lu Zhou, Raphael Nunez-Nateras, Idris T. Ocal, Dora Lam-Himlin, Rosanne Kho

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Study Objective: To describe the histologic characteristics of vaginal tissue in patients with vaginal cuff dehiscence (VCD) after robotic hysterectomy and to compare this group with patients without dehiscence. Design: Retrospective analysis (Canadian Task Force classification II-3). Setting: Academic center. Patients: Seven patients with VCD and 6 patients without VCD. Interventions: Vaginal cuff tissue was obtained from all patients and was stained using hematoxylin-eosin and evaluated for acute and chronic inflammation markers including neutrophils, lymphocytes, and plasma cells. Immunohistochemical staining was performed and evaluated using the semiquantitative method for collagen types I and III, smooth muscle actin, and SM22α (myofibroblast) content. Grading was performed by 4 blinded investigators. The Mann-Whitney test was used to evaluate the 2 groups, and correlation coefficients for interobserver variability. Measurements and Main Results: The VCD group, compared with the non-VCD group, demonstrated significantly greater numbers of neutrophils (1.71 vs 1.0; p = .04), lymphocytes (2.85 vs 1.33; p = .002), and plasma cells (2.2 vs 1.0; p = .001). There was no statistical difference between the groups in amounts of collagen I (1.71 vs 1.27; p = .09) and collagen III (1.66 vs 1.38; p = .37), smooth muscle actin (1.23 vs 1.33; p = .65), and SM22α (1.85 vs 1.27; p = .09). Interobserver variability was low (κ = 0.86; p = .76). Conclusion: Compared with the control group, patients with VCD demonstrated significantly higher levels of acute and chronic inflammatory cells. This finding suggests that a prolonged inflammatory phase may be delaying normal progression to reparation in patients with dehiscence.

Original languageEnglish (US)
Pages (from-to)442-446
Number of pages5
JournalJournal of Minimally Invasive Gynecology
Volume21
Issue number3
DOIs
StatePublished - May 2014

Keywords

  • Histologic characteristics
  • Robotics
  • Vaginal cuff dehiscence

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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    Klauschie, J., Wen, Y., Chen, B., Zhou, L., Nunez-Nateras, R., Ocal, I. T., Lam-Himlin, D., & Kho, R. (2014). Histologic characteristics of vaginal cuff tissue from patients with vaginal cuff dehiscence. Journal of Minimally Invasive Gynecology, 21(3), 442-446. https://doi.org/10.1016/j.jmig.2013.11.013