Histamine-degrading enzymes as cellular markers of acute small bowel allograft rejection.

Alexander Klaus, Helmut Weiss, Justin H. Nguyen, Raimund Margreiter, Peter Obrist, Hubert G. Schwelberger

Research output: Contribution to journalArticle

Abstract

Intestinal histamine-degrading enzymes diamine oxidase (DAO) and histamine N-methyltransferase (HNMT) activities are relatively constant per individual and bowel segment, and they reflect the functional integrity of the intestinal mucosa. It was, therefore, hypothesised that a decrease in these enzymes could be indicative of acute rejection of an intestinal allograft. Enzymatic activities of DAO and HNMT were determined in mucosal biopsies of isogeneic (Lewis-to-Lewis, n=48) and allogeneic (Brown Norway-to-Lewis, n=48) heterotopic small bowel transplants in a rat model at various time periods. Allograft recipients were not given any immunosuppression. While no changes in enzyme activities were observed in isografts up to day 8 following transplantation, significantly reduced activities of both enzymes were found in all allografts 6-8 days after transplantation. Activities of both DAO and HNMT exhibited a strong negative correlation with the histological rejection score ( P

Original languageEnglish (US)
Pages (from-to)474-479
Number of pages6
JournalTransplant International
Volume16
Issue number8
StatePublished - Aug 2003
Externally publishedYes

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Histamine N-Methyltransferase
Amine Oxidase (Copper-Containing)
Histamine
Allografts
Enzymes
Transplantation
Isografts
Intestinal Mucosa
Norway
Immunosuppression
Transplants
Biopsy

ASJC Scopus subject areas

  • Transplantation

Cite this

Klaus, A., Weiss, H., Nguyen, J. H., Margreiter, R., Obrist, P., & Schwelberger, H. G. (2003). Histamine-degrading enzymes as cellular markers of acute small bowel allograft rejection. Transplant International, 16(8), 474-479.

Histamine-degrading enzymes as cellular markers of acute small bowel allograft rejection. / Klaus, Alexander; Weiss, Helmut; Nguyen, Justin H.; Margreiter, Raimund; Obrist, Peter; Schwelberger, Hubert G.

In: Transplant International, Vol. 16, No. 8, 08.2003, p. 474-479.

Research output: Contribution to journalArticle

Klaus, A, Weiss, H, Nguyen, JH, Margreiter, R, Obrist, P & Schwelberger, HG 2003, 'Histamine-degrading enzymes as cellular markers of acute small bowel allograft rejection.', Transplant International, vol. 16, no. 8, pp. 474-479.
Klaus A, Weiss H, Nguyen JH, Margreiter R, Obrist P, Schwelberger HG. Histamine-degrading enzymes as cellular markers of acute small bowel allograft rejection. Transplant International. 2003 Aug;16(8):474-479.
Klaus, Alexander ; Weiss, Helmut ; Nguyen, Justin H. ; Margreiter, Raimund ; Obrist, Peter ; Schwelberger, Hubert G. / Histamine-degrading enzymes as cellular markers of acute small bowel allograft rejection. In: Transplant International. 2003 ; Vol. 16, No. 8. pp. 474-479.
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