Abstract
Intestinal histamine-degrading enzymes diamine oxidase (DAO) and histamine N-methyltransferase (HNMT) activities are relatively constant per individual and bowel segment, and they reflect the functional integrity of the intestinal mucosa. It was, therefore, hypothesised that a decrease in these enzymes could be indicative of acute rejection of an intestinal allograft. Enzymatic activities of DAO and HNMT were determined in mucosal biopsies of isogeneic (Lewis-to-Lewis, n = 48) and allogeneic (Brown Norway-to-Lewis, n = 48) heterotopic small bowel transplants in a rat model at various time periods. Allograft recipients were not given any immunosuppression. While no changes in enzyme activities were observed in isografts up to day 8 following transplantation, significantly reduced activities of both enzymes were found in all allografts 6-8 days after transplantation. Activities of both DAO and HNMT exhibited a strong negative correlation with the histological rejection score (P< 0.01). We can conclude that DAO and HNMT activities in gut mucosa are reliable quantitative markers of acute intestinal allograft rejection in the rat that support histopathological analysis.
Original language | English (US) |
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Pages (from-to) | 572-577 |
Number of pages | 6 |
Journal | Transplant International |
Volume | 16 |
Issue number | 8 |
DOIs | |
State | Published - Aug 1 2003 |
Keywords
- Allograft rejection
- Biochemical markers
- Diamine oxidase
- Histamine N-methyltransferase
- Histamine metabolism
- Rats
- Small bowel transplantation
ASJC Scopus subject areas
- Transplantation