High-throughput genomic analysis in Waldenström's macroglobulinemia

Stéphanie Poulain, Esteban Braggio, Christophe Roumier, Rachid Aijjou, Natacha Broucqsault, Sylvie Galiègue-Zouitina, Salomon Manier, Valérie Soenen, Olivier Nibourel, Patrick Duthilleul, Rafael Fonseca, Xavier Leleu

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Single-nucleotide polymorphism array (SNPa) and array-based comparative genomic hybridization (aCGH) are among the most sensitive genomic high-throughput screening techniques used in the exploration of genetic abnormalities in Waldenström's macroglobulinemia (WM). SNP and aCGH allow the identification of copy number abnormalities (CNA) at the kilobase level thus identifying cryptic genetic abnormalities unseen by lower-resolution approaches such as conventional cytogenetic or fluorescence in situ hybridization (FISH). CNA were identified in nearly 80% of cases by aCGH that delineated in addition minimal altered regions. At gene level, remarkable findings affecting genes involved in the regulation of the NF-kB signaling pathways were identified, such as biallelic inactivation of TNFAIP3 and TRAF3. SNPa also allowed characterization of copy neutral losses such as uniparental disomies (UPD), which is an important and frequent mechanism of gene alteration in cancer cells. Herein, we summarize the current knowledge of WM genomic basis using these high-throughput techniques.

Original languageEnglish (US)
Pages (from-to)106-108
Number of pages3
JournalClinical Lymphoma, Myeloma and Leukemia
Volume11
Issue number1
DOIs
StatePublished - Feb 1 2011

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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    Poulain, S., Braggio, E., Roumier, C., Aijjou, R., Broucqsault, N., Galiègue-Zouitina, S., Manier, S., Soenen, V., Nibourel, O., Duthilleul, P., Fonseca, R., & Leleu, X. (2011). High-throughput genomic analysis in Waldenström's macroglobulinemia. Clinical Lymphoma, Myeloma and Leukemia, 11(1), 106-108. https://doi.org/10.3816/CLML.2011.n.021