TY - JOUR
T1 - High-throughput assay optimization and statistical interpolation of rubella-specific neutralizing antibody titers
AU - Lambert, Nathaniel D.
AU - Pankratz, V. Shane
AU - Larrabee, Beth R.
AU - Ogee-Nwankwo, Adaeze
AU - Chen, Min Hsin
AU - Icenogle, Joseph P.
AU - Poland, Gregory A.
PY - 2014/3
Y1 - 2014/3
N2 - Rubella remains a social and economic burden due to the high incidence of congenital rubella syndrome (CRS) in some countries. For this reason, an accurate and efficient high-throughput measure of antibody response to vaccination is an important tool. In order to measure rubella-specific neutralizing antibodies in a large cohort of vaccinated individuals, a high-throughput immunocolorimetric system was developed. Statistical interpolation models were applied to the resulting titers to refine quantitative estimates of neutralizing antibody titers relative to the assayed neutralizing antibody dilutions. This assay, including the statistical methods developed, can be used to assess the neutralizing humoral immune response to rubella virus and may be adaptable for assessing the response to other viral vaccines and infectious agents.
AB - Rubella remains a social and economic burden due to the high incidence of congenital rubella syndrome (CRS) in some countries. For this reason, an accurate and efficient high-throughput measure of antibody response to vaccination is an important tool. In order to measure rubella-specific neutralizing antibodies in a large cohort of vaccinated individuals, a high-throughput immunocolorimetric system was developed. Statistical interpolation models were applied to the resulting titers to refine quantitative estimates of neutralizing antibody titers relative to the assayed neutralizing antibody dilutions. This assay, including the statistical methods developed, can be used to assess the neutralizing humoral immune response to rubella virus and may be adaptable for assessing the response to other viral vaccines and infectious agents.
UR - http://www.scopus.com/inward/record.url?scp=84896692868&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84896692868&partnerID=8YFLogxK
U2 - 10.1128/CVI.00681-13
DO - 10.1128/CVI.00681-13
M3 - Article
C2 - 24391140
AN - SCOPUS:84896692868
SN - 1556-6811
VL - 21
SP - 340
EP - 346
JO - Clinical and Diagnostic Laboratory Immunology
JF - Clinical and Diagnostic Laboratory Immunology
IS - 3
ER -