High prevalence of tcdC deletion-carrying Clostridium difficile and lack of association with disease severity

Brandon P. Verdoorn, Robert Orenstein, Jon E. Rosenblatt, Lynne M. Sloan, Cathy D. Schleck, William S. Harmsen, Lisa M. Nyre, Robin Patel

Research output: Contribution to journalArticle

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Abstract

We assessed the prevalence of tcdC deletion-carrying Clostridium difficile using a stool polymerase chain reaction (PCR) assay that detects previously described 18- and 39-bp deletions (J. Clin. Microbiol. 2008;46:1996). We divided inpatients into 2 groups, those for whom the assay detected a deletion in tcdC and those for whom no deletion was detected. We compared risk factors (antibiotic use, hospitalization, nursing home stay, immunocompromise, age >65 years), complications (pseudomembranous colitis, toxic megacolon, colonic perforation, colectomy, and intensive care unit admission), duration of antibiotic treatment, and 30-day mortality between the groups. Forty-two of 141 patients had deletion-positive C. difficile. Prior nursing home stay and age >65 years were significantly more common in the deletion-positive group. Other risk factors, complications, antibiotic duration, and mortality did not differ significantly. Deletion-carrying C. difficile was commonly present but not associated with more severe disease and not markedly different in terms of risk factor profile. Severity of disease was relatively low, regardless of the presence or absence of a deletion.

Original languageEnglish (US)
Pages (from-to)24-28
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume66
Issue number1
DOIs
StatePublished - Jan 2010

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Clostridium difficile
Anti-Bacterial Agents
Nursing Homes
Toxic Megacolon
Pseudomembranous Enterocolitis
Mortality
Colectomy
Intensive Care Units
Inpatients
Hospitalization
Polymerase Chain Reaction
Therapeutics

Keywords

  • Clostridium
  • Difficile
  • tcdC

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

High prevalence of tcdC deletion-carrying Clostridium difficile and lack of association with disease severity. / Verdoorn, Brandon P.; Orenstein, Robert; Rosenblatt, Jon E.; Sloan, Lynne M.; Schleck, Cathy D.; Harmsen, William S.; Nyre, Lisa M.; Patel, Robin.

In: Diagnostic Microbiology and Infectious Disease, Vol. 66, No. 1, 01.2010, p. 24-28.

Research output: Contribution to journalArticle

Verdoorn, Brandon P. ; Orenstein, Robert ; Rosenblatt, Jon E. ; Sloan, Lynne M. ; Schleck, Cathy D. ; Harmsen, William S. ; Nyre, Lisa M. ; Patel, Robin. / High prevalence of tcdC deletion-carrying Clostridium difficile and lack of association with disease severity. In: Diagnostic Microbiology and Infectious Disease. 2010 ; Vol. 66, No. 1. pp. 24-28.
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