High-performance liquid chromatographic quantitation of total and lactone 20(S)camptothecin in patients receiving oral 20(S)camptothecin

Farheena Ahmed, Viral Vyas, Ahamed Saleem, Xi Guang Li, Rita Zamek, Adam Cornfield, Paul Haluska, Nageatte Ibrahim, Eric H. Rubin, Elora Gupta

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

We have developed a sensitive high-performance liquid chromatography (HPLC) assay to quantitate total and lactone forms of 20(S)camptothecin (CPT) in human plasma. Lactone and total CPT were extracted using solid-phase extraction and liquid-liquid extraction, respectively. The extracted lactone samples could be stored without immediate HPLC analysis. The two forms of CPT were quantitated by reversed-phase HPLC with fluorescence detection. The extraction efficiencies were about 100% and 92% for the total and lactone forms, respectively. The lower limit of quantitation was 5.74 nM for the two forms. The method was reproducible with a mean interday and intraday variability of 6% for total CPT and 4% and 6%, respectively, for lactone CPT. The assay could effectively quantitate lactone and total CPT in patients receiving single dose and multiple doses of oral CPT.

Original languageEnglish (US)
Pages (from-to)227-233
Number of pages7
JournalJournal of Chromatography B: Biomedical Applications
Volume707
Issue number1-2
DOIs
StatePublished - Apr 10 1998

Keywords

  • 20(S)Camptothecin
  • Lactone 20(S)camptothecin

ASJC Scopus subject areas

  • Chemistry(all)

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