High-performance liquid chromatographic measurement of cyclophosphamide in serum

R. W. Hardy, C. Erlichman, S. J. Soldin

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

The analysis of cyclophosphamide [Af, Af-bis(2-chlorethyl)-tetrahydro-2H-1,3,2-oxazaphosphorin-2-amine-2-oxide] by high-performance liquid chromatography using ultraviolet detection is described. The method will enable measurement of serum concentrations of cyclophosphamide over a period of —24 h after a dose of 150 mg, and requires 1 ml of serum. The between-day precision of the assay at concentrations of 0.3, 1.0, and 15.0 mg/L generated coefficients of variation of 11.8, 12.2, and 7.7%, respectively. Percentages of analytical recovery of cyclophosphamide and internal standard (5-ethyl-5-p-tolylbarbituric acid) were 63 and 73%, respectively. Preliminary data providing the half-life for two patients with normal renal function are presented.

Original languageEnglish (US)
Pages (from-to)313-318
Number of pages6
JournalTherapeutic Drug Monitoring
Volume6
Issue number3
DOIs
StatePublished - Sep 1984

Keywords

  • Cyclophosphamide
  • High-performance liquid chromatography
  • Serum concentrations

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

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