High-performance liquid chromatographic measurement of cyclophosphamide in serum

R. W. Hardy, C. Erlichman, S. J. Soldin

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The analysis of cyclophosphamide [N,N-bis(2-chloroethyl)-tetrahydro-2H-1,3,2-oxazaphosphorin-2-amine-2-oxid e] by high-performance liquid chromatography using ultraviolet detection is described. The method will enable measurement of serum concentrations of cyclophosphamide over a period of ~24 h after a dose of 150 mg, and requires 1 ml of serum. The between-day precision of the assay at concentrations of 0.3, 1.0, and 15.0 mg/L generated coefficients of variation of 11.8, 12.2, and 7.7%, respectively. Percentages of analytical recovery of cyclophosphamide and internal standard (5-ethyl-5-p-tolylbarbituric acid) were 63 and 73%, respectively. Preliminary data providing the half-life for two patients with normal renal function are presented.

Original languageEnglish (US)
Pages (from-to)313-318
Number of pages6
JournalTherapeutic Drug Monitoring
Volume6
Issue number3
StatePublished - 1984
Externally publishedYes

Fingerprint

Cyclophosphamide
Liquids
Serum
High performance liquid chromatography
Amines
Half-Life
Assays
High Pressure Liquid Chromatography
Kidney
Recovery
Acids

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Health, Toxicology and Mutagenesis
  • Pharmacology (medical)
  • Public Health, Environmental and Occupational Health
  • Pharmacology
  • Toxicology

Cite this

Hardy, R. W., Erlichman, C., & Soldin, S. J. (1984). High-performance liquid chromatographic measurement of cyclophosphamide in serum. Therapeutic Drug Monitoring, 6(3), 313-318.

High-performance liquid chromatographic measurement of cyclophosphamide in serum. / Hardy, R. W.; Erlichman, C.; Soldin, S. J.

In: Therapeutic Drug Monitoring, Vol. 6, No. 3, 1984, p. 313-318.

Research output: Contribution to journalArticle

Hardy, RW, Erlichman, C & Soldin, SJ 1984, 'High-performance liquid chromatographic measurement of cyclophosphamide in serum', Therapeutic Drug Monitoring, vol. 6, no. 3, pp. 313-318.
Hardy, R. W. ; Erlichman, C. ; Soldin, S. J. / High-performance liquid chromatographic measurement of cyclophosphamide in serum. In: Therapeutic Drug Monitoring. 1984 ; Vol. 6, No. 3. pp. 313-318.
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