TY - JOUR
T1 - High frequency electrical stimulation promotes expression of extracellular matrix proteins from human astrocytes
AU - Jang, Jin Sung
AU - Choi, Chan Il
AU - Yi, Jiwon
AU - Butters, Kim
AU - Kim, Inyong
AU - Bhagwate, Aditya
AU - Jen, Jin
AU - Chang, Su youne
N1 - Funding Information:
We thank Dr. Penelope Duffy for her help in preparing this manuscript. This study was funded in part by the National Institutes of Health, National Institute of Neurological Disorders and Strokes (NS 88260). John M. Nasseff, Sr. Career Development Award was granted to SYC.
Publisher Copyright:
© 2019, Springer Nature B.V.
PY - 2019/8/15
Y1 - 2019/8/15
N2 - Therapeutic benefits of deep brain stimulation (DBS), a neurosurgical treatment for certain movement disorders and other neurologic conditions, are well documented, but DBS mechanisms remain largely unexplained. DBS is thought to modulate pathological neural activity. However, although astrocytes, the most numerous cell type in the brain, play a significant role in neurotransmission, chemical homeostasis and synaptic plasticity, their role in DBS has not been fully examined. To investigate astrocytic function in DBS, we applied DBS-like high frequency electrical stimulation for 24 h to human astrocytes in vitro and analyzed single cell transcriptome mRNA profile. We found that DBS-like high frequency stimulation negatively impacts astrocyte metabolism and promotes the release of extracellular matrix (matricellular) proteins, including IGFBP3, GREM1, IGFBP5, THBS1, and PAPPA. Our results suggest that astrocytes are involved in the long-term modulation of extra cellular matrix environments and that they may influence persistent cell-to-cell interaction and help maintain neuromodulation over time.
AB - Therapeutic benefits of deep brain stimulation (DBS), a neurosurgical treatment for certain movement disorders and other neurologic conditions, are well documented, but DBS mechanisms remain largely unexplained. DBS is thought to modulate pathological neural activity. However, although astrocytes, the most numerous cell type in the brain, play a significant role in neurotransmission, chemical homeostasis and synaptic plasticity, their role in DBS has not been fully examined. To investigate astrocytic function in DBS, we applied DBS-like high frequency electrical stimulation for 24 h to human astrocytes in vitro and analyzed single cell transcriptome mRNA profile. We found that DBS-like high frequency stimulation negatively impacts astrocyte metabolism and promotes the release of extracellular matrix (matricellular) proteins, including IGFBP3, GREM1, IGFBP5, THBS1, and PAPPA. Our results suggest that astrocytes are involved in the long-term modulation of extra cellular matrix environments and that they may influence persistent cell-to-cell interaction and help maintain neuromodulation over time.
KW - Astrocyte
KW - Deep brain stimulation
KW - Extracellular matrix
KW - Matricellular protein
KW - Single cell transcriptome
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U2 - 10.1007/s11033-019-04890-9
DO - 10.1007/s11033-019-04890-9
M3 - Article
C2 - 31267326
AN - SCOPUS:85069672881
SN - 0301-4851
VL - 46
SP - 4369
EP - 4375
JO - Molecular Biology Reports
JF - Molecular Biology Reports
IS - 4
ER -