High frequency electrical stimulation promotes expression of extracellular matrix proteins from human astrocytes

Jin Sung Jang, Chan Il Choi, Jiwon Yi, Kim Butters, Inyong Kim, Aditya Bhagwate, Jin Jen, Su-Youne Chang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Therapeutic benefits of deep brain stimulation (DBS), a neurosurgical treatment for certain movement disorders and other neurologic conditions, are well documented, but DBS mechanisms remain largely unexplained. DBS is thought to modulate pathological neural activity. However, although astrocytes, the most numerous cell type in the brain, play a significant role in neurotransmission, chemical homeostasis and synaptic plasticity, their role in DBS has not been fully examined. To investigate astrocytic function in DBS, we applied DBS-like high frequency electrical stimulation for 24 h to human astrocytes in vitro and analyzed single cell transcriptome mRNA profile. We found that DBS-like high frequency stimulation negatively impacts astrocyte metabolism and promotes the release of extracellular matrix (matricellular) proteins, including IGFBP3, GREM1, IGFBP5, THBS1, and PAPPA. Our results suggest that astrocytes are involved in the long-term modulation of extra cellular matrix environments and that they may influence persistent cell-to-cell interaction and help maintain neuromodulation over time.

Original languageEnglish (US)
Pages (from-to)4369-4375
Number of pages7
JournalMolecular Biology Reports
Volume46
Issue number4
DOIs
StatePublished - Aug 15 2019

Fingerprint

Deep Brain Stimulation
Extracellular Matrix Proteins
Astrocytes
Electric Stimulation
Neuronal Plasticity
Movement Disorders
Transcriptome
Cell Communication
Synaptic Transmission
Nervous System
Homeostasis
Messenger RNA
Brain
Therapeutics

Keywords

  • Astrocyte
  • Deep brain stimulation
  • Extracellular matrix
  • Matricellular protein
  • Single cell transcriptome

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

High frequency electrical stimulation promotes expression of extracellular matrix proteins from human astrocytes. / Jang, Jin Sung; Choi, Chan Il; Yi, Jiwon; Butters, Kim; Kim, Inyong; Bhagwate, Aditya; Jen, Jin; Chang, Su-Youne.

In: Molecular Biology Reports, Vol. 46, No. 4, 15.08.2019, p. 4369-4375.

Research output: Contribution to journalArticle

Jang, Jin Sung ; Choi, Chan Il ; Yi, Jiwon ; Butters, Kim ; Kim, Inyong ; Bhagwate, Aditya ; Jen, Jin ; Chang, Su-Youne. / High frequency electrical stimulation promotes expression of extracellular matrix proteins from human astrocytes. In: Molecular Biology Reports. 2019 ; Vol. 46, No. 4. pp. 4369-4375.
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