High efficiency adenovirus-mediated gene transfer to human dendritic cells

Allan B Dietz, Stanimir Vuk-Pavlović

Research output: Contribution to journalArticle

180 Citations (Scopus)

Abstract

The interest in the use of human dendritic cells in cancer immunotherapy calls for efficient ex vivo methods of dendritic cell education. To extend the range of methods available, we generated phenotypically characteristic dendritic cells from peripheral blood monocytes incubated with granulocyte- macrophage colony-stimulating factor and interleukin-4 and infected them with an adenovirus containing a humanized version of green fluorescent protein as e marker of gene expression. The levels of expressed protein were high, but they were further increased in combination with cationic liposomes. In comparison to transfection efficiency of the homologous expression plasmid, adenovirus-mediated gene transfer was substantially more efficient. With the aid of liposome-mediated infection, gene transfer into CD83+ dendritic cells was highly effective, resulting in more than 90% of the cells expressing the transgene.

Original languageEnglish (US)
Pages (from-to)392-398
Number of pages7
JournalBlood
Volume91
Issue number2
StatePublished - Jan 15 1998

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Gene transfer
Adenoviridae
Dendritic Cells
Liposomes
Genes
Granulocyte-Macrophage Colony-Stimulating Factor
Green Fluorescent Proteins
Transgenes
Gene expression
Interleukin-4
Immunotherapy
Transfection
Monocytes
Plasmids
Blood
Education
Cells
Gene Expression
Infection
Neoplasms

ASJC Scopus subject areas

  • Hematology

Cite this

High efficiency adenovirus-mediated gene transfer to human dendritic cells. / Dietz, Allan B; Vuk-Pavlović, Stanimir.

In: Blood, Vol. 91, No. 2, 15.01.1998, p. 392-398.

Research output: Contribution to journalArticle

Dietz, AB & Vuk-Pavlović, S 1998, 'High efficiency adenovirus-mediated gene transfer to human dendritic cells', Blood, vol. 91, no. 2, pp. 392-398.
Dietz, Allan B ; Vuk-Pavlović, Stanimir. / High efficiency adenovirus-mediated gene transfer to human dendritic cells. In: Blood. 1998 ; Vol. 91, No. 2. pp. 392-398.
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