High doses of ANP and BNP exacerbate lipolysis in humans and the lipolytic effect of BNP is associated with cardiac triglyceride content in pigs

Emil D. Bartels, Song Guo, Birgitte S. Kousholt, Jens R. Larsen, J. Michael Hasenkam, John C Jr. Burnett, Lars B. Nielsen, Messoud Ashina, Jens P. Goetze

Research output: Contribution to journalArticle

Abstract

Drugs facilitating the cardioprotective effects of natriuretic peptides are introduced in heart failure treatment. ANP and BNP also stimulate lipolysis and increase circulating concentrations of free fatty acids (FFAs); an aspect, however, thought to be confined to primates. We examined the lipolytic effect of natriuretic peptide infusion in healthy young men and evaluated the effect in a porcine model of myocardial ischemia and reperfusion. Six young healthy normotensive men underwent infusion with ANP, BNP, or CNP for 20 min. Blood samples were collected before, during, and after infusion for measurement of FFAs. In a porcine model of myocardial ischemia and reperfusion, animals were infused for 3 h with either BNP (n = 7) or saline (n = 5). Blood samples were collected throughout the infusion period, and cardiac tissue was obtained after infusion for lipid analysis. In humans, ANP infusion dose-dependently increased the FFA concentration in plasma 2.5-10-fold (baseline vs. 0.05 μg/kg/min P < 0.002) and with BNP 1.6–3.5-fold (P = 0.001, baseline vs. 0.02 μg/kg/min) 30 min after initiation of infusion. Infusion of CNP did not affect plasma FFA. In pigs, BNP infusion induced a 3.5-fold increase in plasma FFA (P < 0.0001), which remained elevated throughout the infusion period. Triglyceride content in porcine right cardiac ventricle tissue increased ∼5.5 fold in animals infused with BNP (P = 0.02). Natriuretic peptide infusion has similar lipolytic activity in human and pig. Our data suggest that short-term infusion increases the cardiac lipid content, and that the pig is a suitable model for studies of long-term effects mediated by natriuretic peptides.

Original languageEnglish (US)
Pages (from-to)43-47
Number of pages5
JournalPeptides
Volume112
DOIs
StatePublished - Feb 1 2019

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Lipolysis
Atrial Natriuretic Factor
Natriuretic Peptides
Nonesterified Fatty Acids
Triglycerides
Swine
Myocardial Reperfusion
Plasmas
Animals
Blood
Heart Ventricles
Myocardial Ischemia
Tissue
Lipids
Human Activities
Primates
Heart Failure
Pharmaceutical Preparations

Keywords

  • Free fatty acids
  • Lipolysis
  • Natriuretic peptides

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

Cite this

High doses of ANP and BNP exacerbate lipolysis in humans and the lipolytic effect of BNP is associated with cardiac triglyceride content in pigs. / Bartels, Emil D.; Guo, Song; Kousholt, Birgitte S.; Larsen, Jens R.; Hasenkam, J. Michael; Burnett, John C Jr.; Nielsen, Lars B.; Ashina, Messoud; Goetze, Jens P.

In: Peptides, Vol. 112, 01.02.2019, p. 43-47.

Research output: Contribution to journalArticle

Bartels, Emil D. ; Guo, Song ; Kousholt, Birgitte S. ; Larsen, Jens R. ; Hasenkam, J. Michael ; Burnett, John C Jr. ; Nielsen, Lars B. ; Ashina, Messoud ; Goetze, Jens P. / High doses of ANP and BNP exacerbate lipolysis in humans and the lipolytic effect of BNP is associated with cardiac triglyceride content in pigs. In: Peptides. 2019 ; Vol. 112. pp. 43-47.
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AU - Larsen, Jens R.

AU - Hasenkam, J. Michael

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AB - Drugs facilitating the cardioprotective effects of natriuretic peptides are introduced in heart failure treatment. ANP and BNP also stimulate lipolysis and increase circulating concentrations of free fatty acids (FFAs); an aspect, however, thought to be confined to primates. We examined the lipolytic effect of natriuretic peptide infusion in healthy young men and evaluated the effect in a porcine model of myocardial ischemia and reperfusion. Six young healthy normotensive men underwent infusion with ANP, BNP, or CNP for 20 min. Blood samples were collected before, during, and after infusion for measurement of FFAs. In a porcine model of myocardial ischemia and reperfusion, animals were infused for 3 h with either BNP (n = 7) or saline (n = 5). Blood samples were collected throughout the infusion period, and cardiac tissue was obtained after infusion for lipid analysis. In humans, ANP infusion dose-dependently increased the FFA concentration in plasma 2.5-10-fold (baseline vs. 0.05 μg/kg/min P < 0.002) and with BNP 1.6–3.5-fold (P = 0.001, baseline vs. 0.02 μg/kg/min) 30 min after initiation of infusion. Infusion of CNP did not affect plasma FFA. In pigs, BNP infusion induced a 3.5-fold increase in plasma FFA (P < 0.0001), which remained elevated throughout the infusion period. Triglyceride content in porcine right cardiac ventricle tissue increased ∼5.5 fold in animals infused with BNP (P = 0.02). Natriuretic peptide infusion has similar lipolytic activity in human and pig. Our data suggest that short-term infusion increases the cardiac lipid content, and that the pig is a suitable model for studies of long-term effects mediated by natriuretic peptides.

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