High Detection Rates of Colorectal Neoplasia by Stool DNA Testing With a Novel Digital Melt Curve Assay

Hongzhi Zou, William R. Taylor, Jonathan J. Harrington, Fareeda Taher Nazer Hussain, Xiaoming Cao, Charles Lawrence Loprinzi, Theodore R. Levine, Douglas K. Rex, Dennis Ahnen, Kandice L. Knigge, Peter Lance, Xuan Jiang, David I Smith, David A. Ahlquist

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Abstract

Background & Aims: Current stool DNA tests identify about half of individuals with colorectal cancers and miss most individuals with advanced adenomas. We developed a digital melt curve (DMC) assay to quantify low-abundance mutations in stool samples for detection of colorectal neoplasms and compared this test with other approaches. Methods: We combined a melt curve assay with digital polymerase chain reaction and validated the quantitative range. We then evaluated its ability to detect neoplasms in 2 clinical studies. In study I, stool samples from patients with colorectal tumors with known mutations (KRAS, APC, BRAF, TP53) were assayed. In study II, archived stool samples from patients with advanced adenomas containing known KRAS mutations were assayed, along with controls. Results were compared with those from the stool DNA test PreGenPlus (Exact Sciences, Marlborough, MA), Hemoccult, and HemoccultSensa (both Beckman-Coulter, Fullerton, CA). Results: The DMC assay detected samples in which only 0.1% of target genes were mutated. In study I, the DMC assay detected known mutations in 28 (90%) of 31 tumor samples and 6 (75%) of 8 advanced adenoma samples. In study II, the DMC assay detected 16 (59%) of 27 advanced adenoma samples that contained KRAS mutations, compared with 7% with the Hemoccult, 15% with the HemoccultSensa, and 26% with the PreGenPlus assays (P < .05 for each, compared with the DMC assay); specificities did not differ significantly. Conclusions: The DMC assay has a high level of sensitivity in detecting individuals with colon neoplasms and is better than current stool screening methods in detecting those with advanced adenomas. Further studies are indicated.

Original languageEnglish (US)
Pages (from-to)459-470
Number of pages12
JournalGastroenterology
Volume136
Issue number2
DOIs
StatePublished - Feb 2009

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Adenoma
Mutation
DNA
Colorectal Neoplasms
Neoplasms
Colonic Neoplasms
Polymerase Chain Reaction
Genes

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Zou, H., Taylor, W. R., Harrington, J. J., Hussain, F. T. N., Cao, X., Loprinzi, C. L., ... Ahlquist, D. A. (2009). High Detection Rates of Colorectal Neoplasia by Stool DNA Testing With a Novel Digital Melt Curve Assay. Gastroenterology, 136(2), 459-470. https://doi.org/10.1053/j.gastro.2008.10.023

High Detection Rates of Colorectal Neoplasia by Stool DNA Testing With a Novel Digital Melt Curve Assay. / Zou, Hongzhi; Taylor, William R.; Harrington, Jonathan J.; Hussain, Fareeda Taher Nazer; Cao, Xiaoming; Loprinzi, Charles Lawrence; Levine, Theodore R.; Rex, Douglas K.; Ahnen, Dennis; Knigge, Kandice L.; Lance, Peter; Jiang, Xuan; Smith, David I; Ahlquist, David A.

In: Gastroenterology, Vol. 136, No. 2, 02.2009, p. 459-470.

Research output: Contribution to journalArticle

Zou, H, Taylor, WR, Harrington, JJ, Hussain, FTN, Cao, X, Loprinzi, CL, Levine, TR, Rex, DK, Ahnen, D, Knigge, KL, Lance, P, Jiang, X, Smith, DI & Ahlquist, DA 2009, 'High Detection Rates of Colorectal Neoplasia by Stool DNA Testing With a Novel Digital Melt Curve Assay', Gastroenterology, vol. 136, no. 2, pp. 459-470. https://doi.org/10.1053/j.gastro.2008.10.023
Zou, Hongzhi ; Taylor, William R. ; Harrington, Jonathan J. ; Hussain, Fareeda Taher Nazer ; Cao, Xiaoming ; Loprinzi, Charles Lawrence ; Levine, Theodore R. ; Rex, Douglas K. ; Ahnen, Dennis ; Knigge, Kandice L. ; Lance, Peter ; Jiang, Xuan ; Smith, David I ; Ahlquist, David A. / High Detection Rates of Colorectal Neoplasia by Stool DNA Testing With a Novel Digital Melt Curve Assay. In: Gastroenterology. 2009 ; Vol. 136, No. 2. pp. 459-470.
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AU - Zou, Hongzhi

AU - Taylor, William R.

AU - Harrington, Jonathan J.

AU - Hussain, Fareeda Taher Nazer

AU - Cao, Xiaoming

AU - Loprinzi, Charles Lawrence

AU - Levine, Theodore R.

AU - Rex, Douglas K.

AU - Ahnen, Dennis

AU - Knigge, Kandice L.

AU - Lance, Peter

AU - Jiang, Xuan

AU - Smith, David I

AU - Ahlquist, David A.

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N2 - Background & Aims: Current stool DNA tests identify about half of individuals with colorectal cancers and miss most individuals with advanced adenomas. We developed a digital melt curve (DMC) assay to quantify low-abundance mutations in stool samples for detection of colorectal neoplasms and compared this test with other approaches. Methods: We combined a melt curve assay with digital polymerase chain reaction and validated the quantitative range. We then evaluated its ability to detect neoplasms in 2 clinical studies. In study I, stool samples from patients with colorectal tumors with known mutations (KRAS, APC, BRAF, TP53) were assayed. In study II, archived stool samples from patients with advanced adenomas containing known KRAS mutations were assayed, along with controls. Results were compared with those from the stool DNA test PreGenPlus (Exact Sciences, Marlborough, MA), Hemoccult, and HemoccultSensa (both Beckman-Coulter, Fullerton, CA). Results: The DMC assay detected samples in which only 0.1% of target genes were mutated. In study I, the DMC assay detected known mutations in 28 (90%) of 31 tumor samples and 6 (75%) of 8 advanced adenoma samples. In study II, the DMC assay detected 16 (59%) of 27 advanced adenoma samples that contained KRAS mutations, compared with 7% with the Hemoccult, 15% with the HemoccultSensa, and 26% with the PreGenPlus assays (P < .05 for each, compared with the DMC assay); specificities did not differ significantly. Conclusions: The DMC assay has a high level of sensitivity in detecting individuals with colon neoplasms and is better than current stool screening methods in detecting those with advanced adenomas. Further studies are indicated.

AB - Background & Aims: Current stool DNA tests identify about half of individuals with colorectal cancers and miss most individuals with advanced adenomas. We developed a digital melt curve (DMC) assay to quantify low-abundance mutations in stool samples for detection of colorectal neoplasms and compared this test with other approaches. Methods: We combined a melt curve assay with digital polymerase chain reaction and validated the quantitative range. We then evaluated its ability to detect neoplasms in 2 clinical studies. In study I, stool samples from patients with colorectal tumors with known mutations (KRAS, APC, BRAF, TP53) were assayed. In study II, archived stool samples from patients with advanced adenomas containing known KRAS mutations were assayed, along with controls. Results were compared with those from the stool DNA test PreGenPlus (Exact Sciences, Marlborough, MA), Hemoccult, and HemoccultSensa (both Beckman-Coulter, Fullerton, CA). Results: The DMC assay detected samples in which only 0.1% of target genes were mutated. In study I, the DMC assay detected known mutations in 28 (90%) of 31 tumor samples and 6 (75%) of 8 advanced adenoma samples. In study II, the DMC assay detected 16 (59%) of 27 advanced adenoma samples that contained KRAS mutations, compared with 7% with the Hemoccult, 15% with the HemoccultSensa, and 26% with the PreGenPlus assays (P < .05 for each, compared with the DMC assay); specificities did not differ significantly. Conclusions: The DMC assay has a high level of sensitivity in detecting individuals with colon neoplasms and is better than current stool screening methods in detecting those with advanced adenomas. Further studies are indicated.

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