TY - JOUR
T1 - High-affinity l-arabinose transport operon. Gene product expression and mRNAs
AU - Horazdovsky, Bruce F.
AU - Hogg, Robert W.
N1 - Funding Information:
This investigation was supported by National Institutes of Health grant AM13791 B.F.H. . was supportedi n part by grant HL07415.
PY - 1987/9/5
Y1 - 1987/9/5
N2 - Various portions of the "high-affinity" l-arabinose transport operon were cloned into the plasmid expression vector pKK223-3 and the operon-encoded protein products were identified. The results indicate that three proteins are encoded by this operon. The first is a 33,000 Mr protein that is the product of the promoter-proximal l-arabinose binding protein coding sequence, araF. A 52,000 Mr protein is encoded by sequence 3′ to araF and has been assigned to the araG locus. The sequence 3′ to araG encodes a 31,000 Mr protein that has been assigned to the araH locus. Both the araG and araH gene products are localized in the membrane fraction of the cell, implying a role in the membrane-associated complex of the high-affinity l-arabinose transport system. Nuclease S1 protection studies indicate that two operon message populations are present in the cell, a full-length operon transcript and a seven- to tenfold more abundant binding protein-specific message. The relative abundance of these two message populations correlates with the differential expression of the binding protein and the membrane-associated proteins of the transport system.
AB - Various portions of the "high-affinity" l-arabinose transport operon were cloned into the plasmid expression vector pKK223-3 and the operon-encoded protein products were identified. The results indicate that three proteins are encoded by this operon. The first is a 33,000 Mr protein that is the product of the promoter-proximal l-arabinose binding protein coding sequence, araF. A 52,000 Mr protein is encoded by sequence 3′ to araF and has been assigned to the araG locus. The sequence 3′ to araG encodes a 31,000 Mr protein that has been assigned to the araH locus. Both the araG and araH gene products are localized in the membrane fraction of the cell, implying a role in the membrane-associated complex of the high-affinity l-arabinose transport system. Nuclease S1 protection studies indicate that two operon message populations are present in the cell, a full-length operon transcript and a seven- to tenfold more abundant binding protein-specific message. The relative abundance of these two message populations correlates with the differential expression of the binding protein and the membrane-associated proteins of the transport system.
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U2 - 10.1016/0022-2836(87)90606-1
DO - 10.1016/0022-2836(87)90606-1
M3 - Article
C2 - 2445995
AN - SCOPUS:0023645445
SN - 0022-2836
VL - 197
SP - 27
EP - 35
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 1
ER -