Hexose transporter expression and function in mouse small intestine: Role of diurnal rhythm

Javairiah Fatima, Corey W. Iqbal, Scott G. Houghton, Michael S. Kasparek, Judith A. Duenes, Ye Zheng, Michael G. Sarr

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25 Scopus citations

Abstract

Background: Expression and function of hexose transporters vary diurnally in rat small intestine; however, this subject remains unexplored in mice. Aim: The aim of the study was to investigate the diurnal expression and function of hexose transporters SGLT1, GLUT2, and GLUT5 in mouse small bowel. Methods: Twenty-four c57bl6 mice maintained in a 12-h light/dark room (6 am-6 pm) were sacrificed at 9 am, 3 pm, 9 pm, and 3 am (n∈=∈6 each). In duodenal, jejunal, and ileal mucosa, total cellular mRNA and protein levels were quantitated by real-time PCR and semiquantitative Western blotting, respectively. The everted sleeve technique measured transporter-mediated glucose uptake at 9 am and 9 pm. Results: mRNA expression of SGLT1, GLUT2, and GLUT5 varied diurnally in all three intestinal segments (p∈ ∈0.03). SGLT1, GLUT2, and GLUT5 protein levels varied diurnally in duodenum and jejunum (p∈<∈0.05) but not in ileum. Transporter-mediated glucose uptake was greater at 9 pm than 9 am (p∈ ∈0.04) in all three segments. V max was greater in duodenum (10 vs 6 nmol/cm/s) and jejunum (8 vs 5 nmol/cm/s) at 9 pm compared to 9 am (p∈=∈0.01); K m remained unchanged. Summary: mRNA levels of intestinal hexose transporters varied diurnally. Protein levels peaked 6-12 h later during dark cycle when >70% of food intake occurred; glucose transport followed a similar pattern with increased uptake at 9 pm. Conclusion: Hexose transporter expression and function vary diurnally with nocturnal feeding patterns of mice.

Original languageEnglish (US)
Pages (from-to)634-641
Number of pages8
JournalJournal of Gastrointestinal Surgery
Volume13
Issue number4
DOIs
StatePublished - Apr 1 2009

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Keywords

  • Diurnal rhythm
  • Hexose transporters
  • Mice
  • Small intestine
  • Sugar absorption

ASJC Scopus subject areas

  • Surgery
  • Gastroenterology

Cite this

Fatima, J., Iqbal, C. W., Houghton, S. G., Kasparek, M. S., Duenes, J. A., Zheng, Y., & Sarr, M. G. (2009). Hexose transporter expression and function in mouse small intestine: Role of diurnal rhythm. Journal of Gastrointestinal Surgery, 13(4), 634-641. https://doi.org/10.1007/s11605-008-0776-4