Heterogeneity of human melanoma-associated antigens defined by monoclonal antibodies and conventional xenoantisera

Barry S. Wilson, Neil Elliot Kay, Kohzoh Imai, Soldano Ferrone

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Immunochemical analysis of cultured human melanoma cell detergent extracts and spent culture medium with conventional xenoantisera and monoclonal antibodies identified four types of 94,000 (94K) dalton molecules and two types of high-molecular-weight melanoma-associated antigens by the following characteristics: (1) association with other components, (2) mobility in SDS-PAGE under reducing and nonreducing conditions, (3) antigenicity, and (4) presence in spent culture medium. Conventional xenoantisera were found to contain antibody populations to antigenically distinct structures, some of which have similar apparent molecular weights. Immunodepletion studies showed that the antigenic determinant detected by the monoclonal antibody 225.28S to a high-molecular-weight melanoma-associated antigen was expressed on a subpopulation of the antigens defined by the conventional xenoantiserum #8995. These data prove that antibodies reactive with antigens of similar molecular weight cannot be assumed to identify the same structures, and indicate that tumor-associated antigens may be heterogeneous in the expression of antigenic determinants defined by monoclonal antibodies.

Original languageEnglish (US)
Pages (from-to)69-74
Number of pages6
JournalCancer Immunology Immunotherapy
Volume13
Issue number1
DOIs
StatePublished - Jun 1982
Externally publishedYes

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Melanoma-Specific Antigens
Monoclonal Antibodies
Culture Media
Epitopes
Molecular Weight
Antigens
Antibodies
Neoplasm Antigens
Cell Extracts
Detergents
Polyacrylamide Gel Electrophoresis
Melanoma
Population
HMW-MAA

ASJC Scopus subject areas

  • Oncology
  • Immunology
  • Cancer Research

Cite this

Heterogeneity of human melanoma-associated antigens defined by monoclonal antibodies and conventional xenoantisera. / Wilson, Barry S.; Kay, Neil Elliot; Imai, Kohzoh; Ferrone, Soldano.

In: Cancer Immunology Immunotherapy, Vol. 13, No. 1, 06.1982, p. 69-74.

Research output: Contribution to journalArticle

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