Heterogeneity of adenovirus-mediated transfer of endothelial nitric oxide synthase gene in isolated canine arteries

M. Tsutsui, A. F Y Chen, T. O'Brien, T. B. Crotty, Zvonimir S Katusic

Research output: Contribution to journalArticle

Abstract

The present study was designed to determine efficiency of adenovirus-mediated transfer of endothelial nitric oxide (eNOS) gene in isolated canine basilar, coronary and femoral arteries. Arterial rings were exposed ex vivo (30 min at 37°C) to an adenoviral vector encoding eNOS gene (AdCMVeNOS) or β-galactosidase reporter gene (AdCMVβ-Gal). Twenty-four hours following transduction, transgene expression was evident in adventitia and endothelium. Intensity of β-galactosidase staining and eNOS immunoreactivity was much higher in basilar artery than in coronary and femoral arteries. β-Galactosidase protein levels were significantly higher in basilar artery than in coronary and femoral arteries, In basilar arteries transduced with AdCMVeNOS but not AdCMVβ-Gal, relaxations to low concentrations of bradykinin (1010 - 3×109 mol/L) were significantly augmented. In contrast, in coronary and femoral arteries exposed to AdCMVeNOS, relaxations to bradykinin were not affected. Augmentation of relaxations to bradykinin in basilar artery transduced with AdCMVeNOS was completely blocked by a nitric oxide synthase inhibitor, L-NAME. These results demonstrate higher efficiency of adenovirus-mediated gene transfer in cerebral arteries than in peripheral blood vessels. They also suggest that expression of recombinant eNOS gene could be used to increase local production of NO in cerebral arterial wall.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997

Fingerprint

Galactosidases
Basilar Artery
Nitric Oxide Synthase Type III
Adenoviridae
arteries
Canidae
Femoral Artery
Bradykinin
Arteries
Genes
galactosidases
Coronary Vessels
thighs
dogs
bradykinin
Reporter Genes
genes
Gene transfer
coronary vessels
Gene encoding

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Heterogeneity of adenovirus-mediated transfer of endothelial nitric oxide synthase gene in isolated canine arteries. / Tsutsui, M.; Chen, A. F Y; O'Brien, T.; Crotty, T. B.; Katusic, Zvonimir S.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

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