Heteroduplex generator in analysis of Rh blood group alleles

Jay Stoerker, Carlyn Hurwitz, Nancy C. Rose, Leslie E. Silberstein, W. Edward Highsmith

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

We describe the use of heteroduplex analysis to enhance the resolution of different rhesus-derived (Rh) isotypes. Heteroduplex analysis of different domains of the Rh D and Rh CE loci can be performed to diagnose a variety of blood group incompatibilities. One application of this technique is the ability to test for fetal-maternal blood group incompatibilities during pregnancy. Several new serotype-specific sequence variations were discovered in the mapping of the Rh locus, and used in the construction of artificial heteroduplex generators (HGs). HGs facilitate the resolution of the Rh isotypes by electrophoretic methods.

Original languageEnglish (US)
Pages (from-to)356-360
Number of pages5
JournalClinical chemistry
Volume42
Issue number3
DOIs
StatePublished - 1996

Keywords

  • blood group incompatibilities
  • erythrocytes
  • fetal status
  • genetic variants
  • hemolytic disease of the newborn
  • immunology
  • newborns
  • pregnancy
  • serotype-specific sequence variations

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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  • Cite this

    Stoerker, J., Hurwitz, C., Rose, N. C., Silberstein, L. E., & Highsmith, W. E. (1996). Heteroduplex generator in analysis of Rh blood group alleles. Clinical chemistry, 42(3), 356-360. https://doi.org/10.1093/clinchem/42.3.356