TY - JOUR
T1 - Hepcidin is a key mediator of anemia of inflammation in Crohn's disease
AU - Basseri, Robert J.
AU - Nemeth, Elizabeta
AU - Vassilaki, Maria E.
AU - Basseri, Benjamin
AU - Enayati, Pedram
AU - Shaye, Omid
AU - Bourikas, Leonidas A.
AU - Ganz, Tom
AU - Papadakis, Konstantinos A.
PY - 2013/9/1
Y1 - 2013/9/1
N2 - Anemia often complicates the course of Inflammatory Bowel Disease (IBD). Hepcidin, a liver-produced peptide hormone, is a key mediator of anemia of chronic disease (ACD). We hypothesized that hepcidin is significantly elevated in anemic CD patients and that hepcidin may cause iron restriction and, therefore, mediate ACD. Methods: We enrolled 17 patients with CD and ACD recruited from the Cedars-Sinai IBD Center. Routine blood tests included hemoglobin (Hgb), hematocrit, erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP). Anemia was defined as hemoglobin < 12. g/dL and < 13.5. g/dL, in men and women, respectively. ACD was diagnosed on the basis of a combination of the following: a) normal or elevated ferritin b) lowered serum iron and total iron binding capacity and c) normal percent iron saturation. Serum and urine hepcidin, as well as IL-6 levels were also measured. Patients with documented iron-deficiency anemia were excluded. Results: There was an excellent correlation between urine (expressed as ng/mg of creatinine) and serum hepcidin levels expressed as ng/ml (r = 0.853, p < 0.001). We also found a strong positive correlation between serum hepcidin and ferritin levels (r= 0.723, p= 0.0015). There was a positive correlation between serum hepcidin and IL-6 levels (r= 0.546, p= 0.023). We found a strong negative correlation between serum hepcidin concentrations and Hgb levels (r= 0.528, p= 0.029). Conclusion: We demonstrate that ACD in CD is characterized by high serum IL-6 and hepcidin levels, which negatively correlate with Hgb levels. Our data support the hypothesis that IL-6-driven hepcidin production mediates ACD in patients with CD.
AB - Anemia often complicates the course of Inflammatory Bowel Disease (IBD). Hepcidin, a liver-produced peptide hormone, is a key mediator of anemia of chronic disease (ACD). We hypothesized that hepcidin is significantly elevated in anemic CD patients and that hepcidin may cause iron restriction and, therefore, mediate ACD. Methods: We enrolled 17 patients with CD and ACD recruited from the Cedars-Sinai IBD Center. Routine blood tests included hemoglobin (Hgb), hematocrit, erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP). Anemia was defined as hemoglobin < 12. g/dL and < 13.5. g/dL, in men and women, respectively. ACD was diagnosed on the basis of a combination of the following: a) normal or elevated ferritin b) lowered serum iron and total iron binding capacity and c) normal percent iron saturation. Serum and urine hepcidin, as well as IL-6 levels were also measured. Patients with documented iron-deficiency anemia were excluded. Results: There was an excellent correlation between urine (expressed as ng/mg of creatinine) and serum hepcidin levels expressed as ng/ml (r = 0.853, p < 0.001). We also found a strong positive correlation between serum hepcidin and ferritin levels (r= 0.723, p= 0.0015). There was a positive correlation between serum hepcidin and IL-6 levels (r= 0.546, p= 0.023). We found a strong negative correlation between serum hepcidin concentrations and Hgb levels (r= 0.528, p= 0.029). Conclusion: We demonstrate that ACD in CD is characterized by high serum IL-6 and hepcidin levels, which negatively correlate with Hgb levels. Our data support the hypothesis that IL-6-driven hepcidin production mediates ACD in patients with CD.
KW - Anemia
KW - Anemia of chronic disease
KW - Crohn's disease
KW - Hepcidin
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UR - http://www.scopus.com/inward/citedby.url?scp=84880039507&partnerID=8YFLogxK
U2 - 10.1016/j.crohns.2012.10.013
DO - 10.1016/j.crohns.2012.10.013
M3 - Article
C2 - 23219355
AN - SCOPUS:84880039507
SN - 1873-9946
VL - 7
SP - e286-e291
JO - Journal of Crohn's and Colitis
JF - Journal of Crohn's and Colitis
IS - 8
ER -