Hepatocyte function in a hollow fiber bioreactor

A potential bioartificial liver

Russell A. Shatford, Scott Nyberg, Steven J. Meier, James G. White, William D. Payne, Wei Shou Hu, Frank B. Cerra

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

We have developed a novel hepatocyte loaded hollow fiber bioreactor as a potential bioartificial liver. Freshly harvested rat hepatocytes were entrapped in a three-dimensional gel matrix within hollow fibers in a perfused bioreactor. Gel entrapment allowed cells to be cultured at high density while maintaining tissue-specific function. Hepatocyte function was evaluated in 10 bioreactors, each containing approximately 5 × 107 cells. Oxygen consumption averaged 0.32 pmole/cell/hr, albumin appearance averaged 0.60 pg/cell/hr, and lidocaine clearance (a measure of the P-450 activity) averaged 0.74 pg/cell/hr. Function persisted for the 7 days of the study. Electron microscopy at 7 days showed the distinctive ultrastructure of viable, differentiated hepatocytes: bile canaliculi, intercellular junctions, peroxisomes, abundant mitochondria, and glycogen granules. Maintenance of tissue specific function and ultrastructure suggests that this bioreactor configuration has potential as a device to support patients in liver failure, as well as to study hepatocytes in vitro.

Original languageEnglish (US)
Pages (from-to)549-557
Number of pages9
JournalJournal of Surgical Research
Volume53
Issue number6
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

Fingerprint

Artificial Liver
Bioreactors
Hepatocytes
Gels
Bile Canaliculi
Peroxisomes
Intercellular Junctions
Liver Failure
Lidocaine
Glycogen
Oxygen Consumption
Albumins
Electron Microscopy
Mitochondria
Maintenance
Equipment and Supplies

ASJC Scopus subject areas

  • Surgery

Cite this

Shatford, R. A., Nyberg, S., Meier, S. J., White, J. G., Payne, W. D., Hu, W. S., & Cerra, F. B. (1992). Hepatocyte function in a hollow fiber bioreactor: A potential bioartificial liver. Journal of Surgical Research, 53(6), 549-557. https://doi.org/10.1016/0022-4804(92)90253-V

Hepatocyte function in a hollow fiber bioreactor : A potential bioartificial liver. / Shatford, Russell A.; Nyberg, Scott; Meier, Steven J.; White, James G.; Payne, William D.; Hu, Wei Shou; Cerra, Frank B.

In: Journal of Surgical Research, Vol. 53, No. 6, 01.01.1992, p. 549-557.

Research output: Contribution to journalArticle

Shatford, Russell A. ; Nyberg, Scott ; Meier, Steven J. ; White, James G. ; Payne, William D. ; Hu, Wei Shou ; Cerra, Frank B. / Hepatocyte function in a hollow fiber bioreactor : A potential bioartificial liver. In: Journal of Surgical Research. 1992 ; Vol. 53, No. 6. pp. 549-557.
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