Hepato-renal pathology in Pkd2ws25/- mice, an animal model of autosomal dominant polycystic kidney disease

Angela Stroope, Brynn Radtke, Bing Huang, Tatyana Masyuk, Vicente Torres, Erik Ritman, Nicholas LaRusso

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Polycystic liver diseases, the most important of which are autosomal dominant and autosomal recessive polycystic kidney diseases, are incurable pathological conditions. Animal models that resemble human pathology in these diseases provide an opportunity to study the mechanisms of cystogenesis and to test potential treatments. Here we demonstrate that Pkd2ws25/- mice, an animal model of autosomal dominant polycystic kidney disease, developed hepatic cysts. As assessed by micro-computed tomography scanning of intact livers and by light microscopy of hepatic tissue, hepatic cystic volumes increased from 12.82 ± 3.16% (5- to 8-month-old mice) to 21.58 ± 4.81% (9- to 12-month-old mice). Renal cystogenesis was more severe at early stages of disease: in 5- to 7-month-old mice, cystic volumes represented 40.67 ± 5.48% of kidney parenchyma, whereas in older mice cysts occupied 31.04 ± 1.88% of kidney parenchyma. Mild fibrosis occurred only in liver , and its degree was unchanged with age. Hepatic cysts were lined by single or multiple layers of squamous cholangiocytes. Cystic cholangiocyte cilia were short and malformed, whereas in renal cysts they appeared normal. In Pkd2 ws25/- mice, mitotic and apoptotic indices in both kidney and liver were increased compared with wild-type mice. In conclusion, Pkd2 ws25/- mice exhibit hepatorenal pathology resembling human autosomal dominant polycystic kidney disease and represent a useful model to study mechanisms of cystogenesis and to evaluate treatment options.

Original languageEnglish (US)
Pages (from-to)1282-1291
Number of pages10
JournalAmerican Journal of Pathology
Volume176
Issue number3
DOIs
StatePublished - Mar 2010

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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